Shimohama S, Fujimoto S, Sumida Y, Tanino H
Faculty of Medicine, Kyoto University, Kyoto, Sakyoku, 606, Japan.
Biochem Biophys Res Commun. 1998 Nov 9;252(1):92-6. doi: 10.1006/bbrc.1998.9577.
We have previously examined the involvement of the B cell leukemia-2 gene product (Bcl-2) family proteins (Bcl-2, Bcl-x, Bax, Bak, and Bad) in Alzheimer's disease (AD) and found that Bcl-2, Bcl-x, Bak, and Bad were upregulated. As AD is an aging-associated disease, in the present study we examined the developmental and aging-related changes in Bcl-2 family proteins in the rat brain. Immunoblot analyses of brain extracts from embryonic day 19 (E19) to postnatal 96-week-old rats indicated that the Bcl-2 protein level was highest at E19 and decreased after birth. Bcl-x levels remained high from E19 to 96 weeks. Bax levels were high from E19 to 2 weeks and decreased from 4 weeks onward. Bak levels were highest at E19 and decreased abruptly after birth. Bad levels were high from E19 to 2 weeks and decreased abruptly at 4 weeks. The present results suggest that the expression of each Bcl-2 family protein is differentially regulated during development and aging and that the changes in the senescent brains are different from those observed in AD.
我们之前研究了B细胞白血病-2基因产物（Bcl-2）家族蛋白（Bcl-2、Bcl-x、Bax、Bak和Bad）在阿尔茨海默病（AD）中的作用，发现Bcl-2、Bcl-x、Bak和Bad表达上调。由于AD是一种与衰老相关的疾病，在本研究中，我们检测了大鼠脑中Bcl-2家族蛋白在发育和衰老过程中的变化。对胚胎第19天（E19）至出生后96周龄大鼠的脑提取物进行免疫印迹分析表明，Bcl-2蛋白水平在E19时最高，出生后下降。Bcl-x水平从E19到96周一直保持较高。Bax水平从E19到2周较高，从4周开始下降。Bak水平在E19时最高，出生后急剧下降。Bad水平从E19到2周较高，在4周时急剧下降。目前的结果表明，每个Bcl-2家族蛋白的表达在发育和衰老过程中受到不同的调节，并且衰老大脑中的变化与AD中观察到的变化不同。
