Ekström P, Anzelius M
Department of Zoology, Lund University, Helgonavägen 3, S-223 62, Lund, Sweden.
Brain Res. 1998 Nov 23;812(1-2):179-85. doi: 10.1016/s0006-8993(98)00994-9.
Putative GABAergic elements in the retina of the Atlantic salmon have been identified by immunohistochemistry, utilising polyclonal antisera against gamma-aminobutyric acid (GABA) and the GABA transporter GAT-1. Cell types immunoreactive (ir) for GABA comprise horizontal cells, amacrine cells, displaced amacrine cells in the ganglion cell layer, displaced amacrine cells in the inner plexiform layer (interstitial cells), and Müller cells. In addition, a GABA-immunonegative type of interstitial cell was also identified. In the inner plexiform layer, GABAir fibres were organised in sublayers that were strikingly similar to the sublayering of GAT-1ir fibres. GAT-1ir cell bodies comprise amacrine cells and displaced amacrine cells that may represent a subpopulation of the GABAir ones. In view of the very similar sublayering of GABAir and GAT-ir fibres in the IPL we suggest that a similar type of GABA transporter, that can be recognised with antibodies against rat GAT-1, is present at least in the dendrites of all GABAir amacrine cells but is not expressed in the cell bodies of all GABAir cells.
利用抗γ-氨基丁酸（GABA）和GABA转运体GAT-1的多克隆抗血清，通过免疫组织化学方法在大西洋鲑视网膜中鉴定出了假定的GABA能成分。对GABA呈免疫反应（ir）的细胞类型包括水平细胞、无长突细胞、神经节细胞层中的移位无长突细胞、内网状层中的移位无长突细胞（间质细胞）和穆勒细胞。此外，还鉴定出了一种对GABA免疫阴性的间质细胞类型。在内网状层中，GABA免疫反应性纤维呈亚层状排列，与GAT-1免疫反应性纤维的亚层排列极为相似。GAT-1免疫反应性细胞体包括无长突细胞和移位无长突细胞，它们可能代表GABA免疫反应性细胞的一个亚群。鉴于内网状层中GABA免疫反应性和GAT免疫反应性纤维的亚层排列非常相似，我们认为至少在所有GABA免疫反应性无长突细胞的树突中存在一种可被抗大鼠GAT-1抗体识别的类似类型的GABA转运体，但并非所有GABA免疫反应性细胞的细胞体都表达该转运体。
