Weiss M L, Chowdhury S I
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5602, USA.
Brain Res. 1998 Nov 23;812(1-2):227-41. doi: 10.1016/s0006-8993(98)00950-0.
Retrograde tract tracing studies have indicated that dorsal root ganglion cells from T8 to L2 innervate the rat's left kidney. Electrophysiology studies have indicated that putative second-order sympathetic afferents are found in the dorsal horn at spinal segments T10 to L1 in laminae V-VII. Here, the spread of pseudorabies virus through renal sensory pathways was examined following 2-5 days post-infection (PI) and the virus was located immunocytochemically using a rabbit polyclonal antibody. Two days PI, dorsal root ganglion neurons (first-order sympathetic afferents) were infected with PRV. An average of 1.2, 0.8, 2.1 and 4.4% of the infected dorsal root ganglion neurons were contralateral to the injected kidney at spinal segments T10, T11, T12 and T13, respectively. Four days PI, infected neurons were detected within laminae I and II of the dorsal horn of the caudal thoracic and upper lumbar spinal cord segments. The labeling patterns in the spinal cord are consistent with previous work indicating the location of renal sympathetic sensory pathways. The nodose ganglia were labeled starting 4 days PI, suggesting the involvement of parasympathetic sensory pathways. Five days PI, infected neurons were found in the nucleus tractus solitarius. In the present study, it was unclear whether the infected neurons in the nucleus tractus solitarius are part of sympathetic or parasympathetic afferent pathways or represent a convergence of sensory information. Renal denervation prevented the spread of the virus into the dorsal root ganglia and spinal cord. Sectioning the dorsal roots from T10-L3 blocked viral spread into the spinal cord dorsal horn, but did not prevent infection of neurons in dorsal root ganglion nor did it prevent infection of putative preganglionic neurons in the intermediolateral cell column. The present results indicated that renal afferent pathways can be identified after pseudorabies virus infection of the kidney. Our results suggest that renal afferents travel in sympathetic and parasympathetic nerves and that this information may converge at the NTS.
逆行束路追踪研究表明，胸8至腰2的背根神经节细胞支配大鼠的左肾。电生理学研究表明，在脊髓胸10至腰1节段的背角第V - VII层中发现了假定的二级交感传入神经。在此，在感染后2 - 5天（PI）检查了伪狂犬病病毒通过肾感觉通路的传播，并使用兔多克隆抗体通过免疫细胞化学方法定位病毒。感染后2天，背根神经节神经元（一级交感传入神经）被PRV感染。在胸10、胸11、胸12和胸13节段，分别平均有1.2%、0.8%、2.1%和4.4%的被感染背根神经节神经元位于注射侧肾脏的对侧。感染后4天，在胸段尾端和腰段脊髓背角的第I和第II层内检测到被感染的神经元。脊髓中的标记模式与先前表明肾交感感觉通路位置的研究结果一致。从感染后4天开始，迷走神经节被标记，提示副交感感觉通路参与其中。感染后5天，在孤束核中发现了被感染的神经元。在本研究中，尚不清楚孤束核中被感染的神经元是交感传入通路还是副交感传入通路的一部分，或者是否代表感觉信息的汇聚。肾去神经支配可阻止病毒扩散到背根神经节和脊髓。切断胸10至腰3的背根可阻止病毒扩散到脊髓背角，但不能阻止背根神经节中神经元的感染，也不能阻止中间外侧细胞柱中假定的节前神经元的感染。目前的结果表明，在肾脏感染伪狂犬病病毒后可以识别肾传入通路。我们的结果表明，肾传入神经通过交感神经和副交感神经传导，并且这些信息可能在孤束核汇聚。
