Fukushima K, Sasaki I, Takahashi K i, Naito H, Ogawa H, Sato S, Matsuno S
First Department of Surgery, Tohoku University, School of Medicine, Sendai, Japan.
Digestion. 1998 Nov-Dec;59(6):683-8. doi: 10.1159/000007576.
Although both lipopolysaccharide (LPS) and sodium butyrate are major bacterial products and bioactive chemicals with multiple functions on mucosal cells in the gut, their interaction effects on epithelial cells are not well understood. The purpose of the present study was to investigate whether LPS modulates butyrate-induced and retinoic acid-mediated alkaline phosphatase (ALP) activity of IEC-6 cells - a rat nontransformed small intestinal epithelial cell line. When cells reached confluency, various combinations of sodium butyrate, retinoic acid and LPS were added to the cultures. Cells were then harvested for the measurement of ALP activities. Sodium butyrate, but not retinoic acid or LPS alone, enhanced ALP activity. When LPS was additionally used with butyrate or retinoic acid, synergistic induction of ALP activities was demonstrated. No additive effect for ALP activity was observed when muramyl peptides or N-formylmethionyl-leucyl-phenylalanine was used with these acids. The present study clearly demonstrated that the specific combination of butyrate and LPS synergistically increased ALP activity, an epithelial differentiation-associated marker, of an intestinal epithelial cell line.
尽管脂多糖（LPS）和丁酸钠都是主要的细菌产物和生物活性化学物质，对肠道黏膜细胞具有多种功能，但它们对上皮细胞的相互作用影响尚未得到充分了解。本研究的目的是调查LPS是否调节丁酸盐诱导的以及视黄酸介导的IEC-6细胞（一种大鼠未转化的小肠上皮细胞系）碱性磷酸酶（ALP）活性。当细胞达到汇合状态时，将丁酸钠、视黄酸和LPS的各种组合添加到培养物中。然后收获细胞以测量ALP活性。单独的丁酸钠可增强ALP活性，而视黄酸或LPS则不能。当LPS与丁酸盐或视黄酸一起使用时，可证明对ALP活性有协同诱导作用。当胞壁酰肽或N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸与这些酸一起使用时，未观察到对ALP活性的相加效应。本研究清楚地表明，丁酸盐和LPS的特定组合可协同增加肠道上皮细胞系中与上皮分化相关的标志物ALP的活性。
