Yang M Q, Kjellén E, Håkansson C H, Palmegren M
Department of Oncology, University Hospital, Lund, Sweden.
Scanning Microsc. 1996;10(3):821-31; discussion 831-2.
In order to examine the effect of radiation on growing tissue, especially the fibroblasts and their end-product, the collagen fibres, tails from 24 mice were irradiated at an age of 8 days with 20 Gy and 30 Gy (60Co). Tails from 18 animals served as controls. Six mice from each group were sacrificed on day 8, 20 and 30. Transmission electron microscopy was used to examine the fibroblasts and the collagen fibrils. Non-irradiated fibroblasts had a nucleus rich in chromatin and an abundant endoplasmic reticulum with cisternae and condensing vacuoles. On day 20, approximately 50%, and on day 30, 25% of the fibroblasts irradiated with 30 Gy had a sparse endoplasmic reticulum pointing to a reduction of protein synthesis. While, on day 20, the fibrils irradiated with 20 Gy and with 30 Gy had significantly larger diameters compared to the controls, on day 30, the irradiated fibrils had a notably smaller diameter compared to the controls; 30 Gy-fibrils were larger than the 20 Gy-fibrils on both days. On day 20, the binding mean value of the 30 Gy-fibrils exceeded that of the controls and was significantly higher than that of the 20 Gy-fibrils, which was lower, though not significantly, than the controls. On day 30, the banding mean value of the 30 Gy-fibrils was notably lower than the control; and the value of the 20 Gy-fibrils was significantly lower than that of the 30 Gy-fibrils. The results are explained as an edema together with an inhibitory effect on the protein synthesis of the fibroblasts caused by the irradiation. This deduction is further supported by light microscopy of the tails.
为了研究辐射对生长组织的影响，特别是对成纤维细胞及其终产物胶原纤维的影响，选取24只8日龄小鼠的尾巴，分别用20 Gy和30 Gy（钴-60）进行照射。18只动物的尾巴作为对照。每组6只小鼠分别在第8天、第20天和第30天处死。采用透射电子显微镜观察成纤维细胞和胶原纤维。未照射的成纤维细胞有一个富含染色质的细胞核和丰富的内质网，内质网有扁平囊和浓缩泡。在第20天，接受30 Gy照射的成纤维细胞中约50%，在第30天，25%的成纤维细胞内质网稀疏，表明蛋白质合成减少。在第20天，接受20 Gy和30 Gy照射的纤维与对照组相比，直径明显更大；在第30天，照射后的纤维直径与对照组相比明显更小；在这两天，30 Gy照射的纤维都比20 Gy照射的纤维大。在第20天，30 Gy照射的纤维的结合平均值超过对照组，且显著高于20 Gy照射的纤维，20 Gy照射的纤维虽低于对照组但差异不显著。在第30天，30 Gy照射的纤维的条带平均值明显低于对照组；20 Gy照射的纤维的值明显低于30 Gy照射的纤维。结果解释为辐射引起的水肿以及对成纤维细胞蛋白质合成的抑制作用。尾巴的光学显微镜检查进一步支持了这一推断。
