Burger H G, Groome N P, Robertson D M
Prince Henry's Institute of Medical Research, Monash Medical Center, Clayton, Victoria, Australia.
J Clin Endocrinol Metab. 1998 Nov;83(11):4167-9. doi: 10.1210/jcem.83.11.5282.
To ascertain whether changes in the concentrations of the dimeric inhibins A and/or B (INH-A and INH-B) contributed to the previously described dose-dependent increase in immunoreactive inhibin (INH) in response to FSH during the follicular phase of the human menstrual cycle, both dimers were measured by specific two-site assays in stored serum samples from regularly cycling normal volunteers who had received saline as a control (n = 5) or FSH [100 IU (n = 6) or 200 IU (n = 5)] between days 3-5 of the menstrual cycle. Both INH-A and INH-B showed a dose-dependent increase in response to administered FSH; INH-A rose from 13.5 to 35.9 ng/L (P < 0.01), and INH-B rose from 77.8 to 205 ng/L (P < 0.05) at 36 h after 200 IU FSH. Highly significant correlations were observed between INH and each of the specific inhibin dimers (A: r = 0.79, P < 0.001; B: r = 0.76, P < 0.001), and the responses of the two dimers were also highly correlated (r = 0.59, P < 0.001). The response of each inhibin was also highly correlated with the response of serum estradiol (A: r = 0.45, P < 0.001; B: r = 0.40, P < 0.001). When analyzed by ANOVA, the INH response of INH-B was significantly above the control value at 36 h after treatment with both 100 and 200 IU FSH, whereas the response of INH-A was significant only at 200 IU. It is concluded that the concentrations of both dimeric INH-A and INH-B are stimulated by increases in FSH within the physiological range in the follicular phase of the human menstrual cycle and that both contribute to the previously observed rise in INH.
为了确定二聚体抑制素A和/或B（INH-A和INH-B）浓度的变化是否导致先前所述的在人类月经周期卵泡期对促卵泡激素（FSH）反应中免疫反应性抑制素（INH）呈剂量依赖性增加，在月经周期第3至5天接受生理盐水作为对照（n = 5）或FSH [100 IU（n = 6）或200 IU（n = 5）]的规律月经周期正常志愿者的储存血清样本中，通过特异性双位点分析法测量了这两种二聚体。INH-A和INH-B对给予的FSH均表现出剂量依赖性增加；200 IU FSH后36小时，INH-A从13.5 ng/L升至35.9 ng/L（P < 0.01），INH-B从77.8 ng/L升至205 ng/L（P < 0.05）。观察到INH与每种特异性抑制素二聚体之间存在高度显著相关性（A：r = 0.79，P < 0.001；B：r = 0.76，P < 0.001），并且两种二聚体的反应也高度相关（r = 0.59，P < 0.001）。每种抑制素的反应与血清雌二醇的反应也高度相关（A：r = 0.45，P < 0.001；B：r = 0.40，P < 0.001）。通过方差分析（ANOVA）分析时，100和200 IU FSH治疗后36小时，INH-B的INH反应显著高于对照值，而INH-A的反应仅在200 IU时显著。结论是，在人类月经周期卵泡期的生理范围内，FSH的增加刺激了二聚体INH-A和INH-B的浓度，并且两者都促成了先前观察到的INH升高。
