Chandran S, Svendsen C, Compston A, Scolding N
MRC Cambridge Centre for Brain Repair, University of Cambridge, United Kingdom.
Glia. 1998 Dec;24(4):382-9. doi: 10.1002/(sici)1098-1136(199812)24:4<382::aid-glia3>3.0.co;2-5.
The origin of oligodendrocytes in the developing rodent spinal cord has not been fully established, with some evidence that oligodendrocyte progenitors arise exclusively from the ventral neuroepithelium, other studies suggesting that both halves of the spinal cord have oligodendrogenic potential. One way of exploring this issue is to study more primitive oligodendrocyte precursors. Although specific markers are not available, their presence may be inferred using mitogens such as EGF and FGF-2, which stimulate the proliferation of immature neuroepithelial cells, and subsequently studying their differentiation into lineage restricted cells. We used this approach to assess whether the dorsal embryonic rodent spinal cord has the intrinsic potential for oligodendrocyte formation at E14. We confirm that significant numbers of oligodendrocytes and their immediate (A2B5+) precursors are present only in the ventral spinal cord of the E14 rodent, but following exposure to EGF and FGF-2, significant numbers of oligodendrocytes and A2B5+ precursor cells also develop from isolated E14 dorsal derived cells without interaction from the ventral spinal cord. In addition, bromodeoxyuridine studies demonstrate that isolated dorsal derived cells proliferate and express A2B5 following exposure to EGF and FGF-2. The observation that from E14, the dorsal cord already has latent oligodendrogenic potential provides an alternative mechanism for oligodendrocyte formation to ventro-dorsal migration of oligodendrocyte precursors.
在发育中的啮齿动物脊髓中，少突胶质细胞的起源尚未完全明确。有证据表明少突胶质前体细胞仅起源于腹侧神经上皮，而其他研究则表明脊髓的两半都具有少突胶质细胞生成潜能。探索这个问题的一种方法是研究更原始的少突胶质细胞前体。尽管没有可用的特异性标志物，但可以通过使用有丝分裂原（如表皮生长因子和碱性成纤维细胞生长因子-2）来推断它们的存在，这些有丝分裂原可刺激未成熟神经上皮细胞的增殖，随后研究它们向谱系受限细胞的分化。我们采用这种方法来评估E14期胚胎啮齿动物脊髓背侧是否具有形成少突胶质细胞的内在潜能。我们证实，大量的少突胶质细胞及其直接前体（A2B5+）仅存在于E14期啮齿动物的脊髓腹侧，但在暴露于表皮生长因子和碱性成纤维细胞生长因子-2后，大量的少突胶质细胞和A2B5+前体细胞也从分离的E14期背侧来源细胞中发育出来，且无需与脊髓腹侧相互作用。此外，溴脱氧尿苷研究表明，分离的背侧来源细胞在暴露于表皮生长因子和碱性成纤维细胞生长因子-2后会增殖并表达A2B5。从E14期开始，脊髓背侧就已经具有潜在的少突胶质细胞生成潜能这一观察结果，为少突胶质细胞形成提供了一种不同于少突胶质前体细胞腹侧到背侧迁移的替代机制。
