Ji C M, Royce F H, Truong U, Plopper C G, Singh G, Pinkerton K E
Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
Am J Physiol. 1998 Nov;275(5):L870-6. doi: 10.1152/ajplung.1998.275.5.L870.
We have previously demonstrated that aged and diluted sidestream cigarette smoke (ADSS) alters the development of bronchiolar epithelial cells in postnatal animals (C. M. Ji, C. G. Plopper, H. P. Witschi, and K. E. Pinkerton. Am. J. Respir. Cell Mol. Biol. 11: 312-320, 1994). This study was designed to examine the effects of maternal exposure to ADSS on the development of fetal Clara cells in rats with Clara cell 10-kDa protein (CC10; also designated Clara cell secretory protein) and CC10 mRNA as differentiation markers. Immunohistochemistry, Northern blots, and in situ hybridization were used to determine the abundance and distribution of CC10 at gestational days 14, 18, and 21. CC10 and CC10 mRNA were absent at gestational day 14 but were detectable at gestational day 18 and further increased by gestational day 21. Maternal exposure to ADSS was found to significantly increase fetal expression of CC10 and CC10 mRNA by gestational day 21 but not by gestational day 14 or 18. These findings demonstrate that in utero exposure to ADSS alters the normal developmental expression of CC10 in the fetal rat lung.
我们之前已经证明，老化和稀释的侧流香烟烟雾（ADSS）会改变出生后动物细支气管上皮细胞的发育（C.M. Ji、C.G. Plopper、H.P. Witschi和K.E. Pinkerton。《美国呼吸细胞与分子生物学杂志》11: 312 - 320，1994年）。本研究旨在以克拉拉细胞10 kDa蛋白（CC10；也称为克拉拉细胞分泌蛋白）和CC10 mRNA作为分化标志物，研究母体暴露于ADSS对大鼠胎儿克拉拉细胞发育的影响。采用免疫组织化学、Northern印迹和原位杂交技术来确定妊娠第14、18和21天CC10的丰度和分布。妊娠第14天时不存在CC10和CC10 mRNA，但在妊娠第18天时可检测到，到妊娠第21天时进一步增加。发现母体暴露于ADSS会使妊娠第21天胎儿CC10和CC10 mRNA的表达显著增加，但在妊娠第14天或18天时不会。这些发现表明，子宫内暴露于ADSS会改变胎鼠肺中CC10的正常发育表达。
