Zhao S F, Li W, Dornadula G, Dicker D, Hoxie J, Peiper S C, Pomerantz R J, Duan L X
Dorrance H Hamilton Laboratories, Center for Human Virology, Department of Medicine, Division of Infectious Diseases, Philadelphia, PA 19107-6799, USA.
J Infect Dis. 1998 Dec;178(6):1623-34. doi: 10.1086/314506.
There exist at least two major coreceptors for human immunodeficiency virus (HIV)-1 entry into target cells, the CXCR-4 and CCR-5 chemokine receptors for T lymphocyte-tropic and macrophage-tropic strains of HIV-1, respectively. Highly purified human CD34 cells derived from umbilical cord blood were shown not to express CD4, CXCR-4, and CCR-5 on their cell membranes, as analyzed by immunofluorescent staining and flow cytometric analyses. However, expression of these molecules was inducible when highly purified CD34 cells underwent proliferation and differentiation along myeloid cell lineages, in the presence of suitable cocktails of hematopoietic growth factors. HIV-1 infectivity studies showed that macrophage-tropic strains of HIV-1 could efficiently infect differentiated CD34 cells. T lymphocyte-tropic strains could not infect CD34 cells before or after induction of receptors and coreceptors. These data suggest that HIV-1 infection of CD34 cells and their progeny depends on membrane expression of the critical CD4 receptor, as well as certain chemokine coreceptors.
人类免疫缺陷病毒（HIV）-1进入靶细胞至少存在两种主要的共受体，即CXCR-4和CCR-5趋化因子受体，分别针对HIV-1的T淋巴细胞嗜性株和巨噬细胞嗜性株。通过免疫荧光染色和流式细胞术分析表明，源自脐带血的高度纯化的人CD34细胞在其细胞膜上不表达CD4、CXCR-4和CCR-5。然而，当高度纯化的CD34细胞在合适的造血生长因子混合物存在下沿髓系细胞谱系进行增殖和分化时，这些分子的表达是可诱导的。HIV-1感染性研究表明，HIV-1的巨噬细胞嗜性株可有效感染分化的CD34细胞。T淋巴细胞嗜性株在诱导受体和共受体之前或之后均不能感染CD34细胞。这些数据表明，CD34细胞及其后代的HIV-1感染取决于关键CD4受体以及某些趋化因子共受体的膜表达。
