Carr J M, Ramshaw H S, Li P, Burrell C J
National Centre for HIV Virology Research, Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J Gen Virol. 1998 Jan;79 ( Pt 1):71-5. doi: 10.1099/0022-1317-79-1-71.
Highly purified (>98%) CD34+ cells directly after isolation (D0) or 2 weeks in culture (D14) were CD4+ and contained mRNA for the T-tropic HIV co-receptor, CXCR-4, and minor co-receptor, CCR-2B. D14 but not D0 cells were RT-PCR positive for mRNA for the major M-tropic human immunodeficiency virus (HIV) co-receptor, CCR-5, and potential co-receptor, CCR-1. D14 and D0 cells were susceptible to T- (HXB2) and M-tropic HIV (Bal), showing greater virus production with Bal than HXB2, and with higher virus production levels in D14 compared to D0 cells. Seven days post-infection of D0 cells Bal DNA was present in CD14bright and CD14- fractions, suggesting D0 infection of diverse progenitor types. HXB2 DNA was detected in CD14bright cells alone indicating D0 infection of monocyte progenitors only. It is concluded that CD34+ cells and cultured derivatives are susceptible to M- and T-tropic HIV and this correlates in part with co-receptor expression at the mRNA level.
分离后即刻（D0）或培养2周（D14）的高度纯化（>98%）的CD34+细胞为CD4+，含有T嗜性HIV共受体CXCR-4和次要共受体CCR-2B的mRNA。D14细胞而非D0细胞的主要M嗜性人类免疫缺陷病毒（HIV）共受体CCR-5和潜在共受体CCR-1的mRNA的RT-PCR检测呈阳性。D14和D0细胞对T嗜性（HXB2）和M嗜性HIV（Bal）敏感，与HXB2相比，Bal感染时病毒产生更多，且与D0细胞相比，D14细胞的病毒产生水平更高。D0细胞感染Bal 7天后，CD14bright和CD14-亚群中存在Bal DNA，提示D0细胞可感染多种祖细胞类型。仅在CD14bright细胞中检测到HXB2 DNA，表明D0细胞仅感染单核细胞祖细胞。结论是，CD34+细胞及其培养衍生物对M嗜性和T嗜性HIV敏感，这部分与mRNA水平上共受体的表达相关。
