Slonim A, Walker E S, Mishori E, Porat N, Dagan R, Yagupsky P
Clinical Microbiology Laboratory and Pediatric Infectious Disease Unit, Soroka Medical Center, Ben-Gyruion University of the Negev, Beer-Sheva, Israel.
J Infect Dis. 1998 Dec;178(6):1843-6. doi: 10.1086/314488.
Fifty Kingella kingae organisms, isolated from tonsillar cultures of day care center attendees during an 11-month period, and 60 isolates derived from epidemiologically unrelated individuals, including 19 isolates from respiratory carriers and 41 isolates from patients with invasive infections, were typed by immunoblotting, pulsed-field gel electrophoresis, and ribotyping. One strain, defined by unique immunoblotting, pulsed-field gel electrophoresis, and ribotyping patterns, represented 14 day care isolates (28%) and was frequently isolated during the first half of the follow-up period; a second strain represented 23 (46%) isolates and prevailed during the last 5 months. Children frequently carried the same strain continuously or intermittently for weeks or months, when it was replaced by a new strain. Epidemiologically unrelated organisms showed greater variability, and no strain represented >5% of isolates. The present results support person-to-person transmission of K. kingae among young children in the day care setting.
在11个月的时间里，从日托中心儿童的扁桃体培养物中分离出50株金氏金杆菌，另外从流行病学上无关联的个体中分离出60株，包括19株呼吸道带菌者的分离株和41株侵袭性感染患者的分离株，采用免疫印迹法、脉冲场凝胶电泳法和核糖体分型法对这些菌株进行分型。一株菌株，通过独特的免疫印迹、脉冲场凝胶电泳和核糖体分型模式确定，占14株日托中心分离株（28%），且在随访期的前半期经常分离到；另一株菌株占23株（46%）分离株，在最后5个月占优势。儿童经常连续或间歇性地携带同一菌株数周或数月，之后被新菌株取代。流行病学上无关联的菌株表现出更大的变异性，没有任何一株菌株占分离株的比例超过5%。目前的结果支持金氏金杆菌在日托环境中的幼儿之间通过人传人传播。
