Baldi A, Esposito V, De Luca A, Fu Y, Meoli I, Giordano G G, Caputi M, Baldi F, Giordano A
Departments of Pathology, Institute for Cancer Research and Molecular Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA.
Clin Cancer Res. 1997 Oct;3(10):1691-7.
Two proteins, p107 and pRb2/p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. Using immunocytochemical techniques, we examined a variety of normal human tissues for the expression of pRb2/p130 and p107. Both proteins were expressed ubiquitously, although a different tissue distribution and/or level of expression were found in various organs. Terminally differentiated cells, such as neurons and skeletal muscle, showed high expression levels for Rb2/p130, whereas p107 was expressed at higher levels in other cell types such as epithelia of the breast and prostate. We then examined the expression pattern of Rb2/p130 in 158 specimens of human lung cancer and found an inverse correlation between the histological grading of the tumors, the development of metastasis, and the level of expression of Rb2/p130.
两种蛋白质，p107和pRb2/p130，它们在结构和功能上与视网膜母细胞瘤基因（pRb）的产物相似，利用它们通过一个称为“口袋结构域”的特定区域与DNA肿瘤病毒的转化蛋白结合的能力被克隆出来。与pRb一样，这两种蛋白质在生长控制中都起着重要作用。我们使用免疫细胞化学技术检测了多种正常人体组织中pRb2/p130和p107的表达情况。两种蛋白质均广泛表达，不过在不同器官中发现了不同的组织分布和/或表达水平。终末分化细胞，如神经元和骨骼肌，显示出较高水平的Rb2/p130表达，而p107在其他细胞类型如乳腺和前列腺上皮中表达水平较高。然后我们检测了158例人肺癌标本中Rb2/p130的表达模式，发现肿瘤的组织学分级、转移的发生与Rb2/p130的表达水平之间呈负相关。
