Kobari M, Kullenberg B, Björkman A, Matsuno S, Ihse I, Axelson J
Department of Surgery, University Hospital, Lund, Sweden.
Int J Pancreatol. 1998 Oct;24(2):85-95. doi: 10.1007/BF02788565.
Epidermal growth factor (EGF) increased the cell number of the two pancreatic cancer cell lines, MiaPaCa-2 and LN-36, in vitro. A blockade of the EGF-R tyrosine kinase with tyrphostin was more efficient in reducing the cell number than inhibiting receptor antibodies. IGF-1 increased the cell number, and blockade of the IGF-1-R initially decreased the cell number that later was followed by an increase in LN-36.
BACKGROUND/AIM: The receptors and ligands of EGF and insulin-like growth factor-1 (IGF-1) are overexpressed in pancreatic cancer tissue. The aim of the present experiments was to study the effects of EGF and IGF-1 on the cell number in two pancreatic cancer cell lines.
MiaPaCa-2 cells were grown in 0.2% fetal calf serum (FCS) and the newly established LN-36 cells in serum-free medium (SFM). The cell number was measured with the XTT method. The effects of EGF and IGF-1 were studied in combination with inhibiting receptor antibodies and an EGF-R-specific tyrosine kinase inhibitor, tyrphostin B56.
MiaPaCa-2 responded with increased cell number to stimulation with EGF, and at 10(-8) M or higher concentrations a dose-response pattern was seen. Administration of B56 to MiaPaCa-2 decreased the cell number by 87%. The inhibiting EGF-R-Ab only inhibited EGF-induced increase in cell number. IGF-1 doubled the cell number of MiaPaCa-2 and increased the cell growth induced by EGF. The inhibiting IGF-1-R-Ab reduced the cell number by 10%. The LN-36 cell line responded to EGF with an increased cell number with a maximum at 5 x 10(-9) M after 96 h. B56 reduced the cell number by 90% at 10(-5) M, with less effect during stimulation with EGF. In contrast to B56, the inhibiting EGF-R-Ab in the same experiment did not reduce the cell number. LN-36 responded to IGF-1 with an increased cell number, but EGF-stimulated growth was not influenced. The inhibiting IGF-1-R-Ab reduced the cell number and suppressed the IGF-1 stimulated increase after 24 h and later it induced an increased cell number.
表皮生长因子（EGF）在体外增加了两种胰腺癌细胞系MiaPaCa - 2和LN - 36的细胞数量。用 tyrphostin阻断EGF - R酪氨酸激酶在减少细胞数量方面比抑制受体抗体更有效。胰岛素样生长因子-1（IGF - 1）增加了细胞数量，阻断IGF - 1 - R最初减少了细胞数量，随后LN - 36细胞数量增加。
背景/目的：EGF和胰岛素样生长因子-1（IGF - 1）的受体和配体在胰腺癌组织中过表达。本实验的目的是研究EGF和IGF - 1对两种胰腺癌细胞系细胞数量的影响。
MiaPaCa - 2细胞在0.2%胎牛血清（FCS）中培养，新建立的LN - 36细胞在无血清培养基（SFM）中培养。用XTT法测量细胞数量。研究了EGF和IGF - 1与抑制受体抗体及一种EGF - R特异性酪氨酸激酶抑制剂tyrphostin B56联合使用的效果。
MiaPaCa - 2细胞对EGF刺激的反应是细胞数量增加，在10^(-8) M或更高浓度时呈现剂量反应模式。向MiaPaCa - 2细胞施用B56使细胞数量减少了87%。抑制性EGF - R抗体仅抑制EGF诱导的细胞数量增加。IGF - 1使MiaPaCa - 2细胞数量增加一倍，并增强了EGF诱导的细胞生长。抑制性IGF - 1 - R抗体使细胞数量减少了10%。LN - 36细胞系对EGF的反应是细胞数量增加，96小时后在5×10^(-9) M时达到最大值。10^(-5) M的B56使细胞数量减少了90%，在EGF刺激期间作用较小。与B56相反，同一实验中的抑制性EGF - R抗体并未减少细胞数量。LN - 36细胞对IGF - 1的反应是细胞数量增加，但EGF刺激的生长未受影响。抑制性IGF - 1 - R抗体减少了细胞数量，并在24小时后抑制了IGF - 1刺激的细胞数量增加，但随后又诱导细胞数量增加。
