Computer simulation as a tool in evaluating intracellular spatial arrangement of an organelle using random section data.

A random profile of a cell gives a highly biased presentation of the location of various organelles within an intracellular space. The technique combining mathematical modeling and computer simulation presented here is aimed to overcome this bias and interpret intracellular spatial arrangement of an organelle using two-dimensional (2-D) observations from random sections. It allows to simulate random sectioning of a cell whose shape approximates to an ellipsoid of rotation, and to obtain the coordinates for the center of an organelle profile located within a cell profile. The pilot study was performed to investigate the influence of different three-dimensional (3-D) scattering patterns of an organelle on the coordinates of an organelle profile's center. Computer tests were carried out on a personal computer using the original software written in Pascal. It was ascertained that statistical properties of a sample of organelle profile's center coordinates allow for a quantitative estimation of some 3-D features, including the position of an organelle with respect to a cell center and specific characteristics of an organelle position (e.g., its fixity or randomness).