Baselt R C, Stewart C B
Res Commun Chem Pathol Pharmacol. 1976 Oct;15(2):351-60.
A method has been developed for the analysis of unbound salicylate by membrane cone ultrafiltration of either whole blood or plasma with quantitation by spectrofluorometry. The method was used to study the distribution of salicylate in whole blood over a wide range of concentrations. It was found that at a concentration of 50 ug/ml in whole blood only 9% of the salicylate is present as free drug in the plasma water, while at a concentration of 500 ug/ml, 23% is free. At concentrations exceeding 500 ug/ml the plasma protein binding sites appear to be fully satrated since the free drug concentration increases linearly with respect to the whole blood concentration. Changes in hematocrit have a relatively minor effect on the ultrafiltrate salicylate concentration. The blood/plasma salicylate concentration ratio was found to vary widely over the blood salicylate range studied, 50-1000 ug/ml.
已开发出一种通过膜锥超滤全血或血浆来分析游离水杨酸，并采用荧光光谱法定量的方法。该方法用于研究水杨酸在全血中广泛浓度范围内的分布情况。结果发现，在全血浓度为50微克/毫升时，血浆水中只有9%的水杨酸以游离药物形式存在，而在浓度为500微克/毫升时，23%是游离的。当浓度超过500微克/毫升时，血浆蛋白结合位点似乎已完全饱和，因为游离药物浓度相对于全血浓度呈线性增加。血细胞比容的变化对超滤水杨酸浓度的影响相对较小。在所研究的血清水杨酸浓度范围（50 - 1000微克/毫升）内，血/浆水杨酸浓度比变化很大。
