Oboh H A, Muzquiz M, Burbano C, Cuadrado C, Pedrosa M M, Ayet G, Osagie A U
Area de Tecnología de Alimentos, SGIT-INIA, Madrid, Spain.
J Chromatogr A. 1998 Oct 9;823(1-2):307-12. doi: 10.1016/s0021-9673(98)00542-1.
Six underutilized legume seeds grown in Nigeria namely, red and white lima beans, brown and cream pigeon pea, African yam bean and jackbean were analysed for different anti-nutritional factors Sojasapogenol B was identified as the predominant sapogenol in lima beans and jackbeans by capillary gas chromatography. The content of total inositol phosphates and individual inositol phosphates (IP6, IP5, IP4 and IP3) were analysed by ion-pair HPLC, being in the range of other legumes. Trace quantities of lupanine were identified as the alkaloid in jackbean. alpha-Galactosides were present in all the legume seeds, stachyose being the predominant galactoside in lima beans, African yam bean and jackbean, and verbascose in pigeon pea. The haemagglutinating activity was estimated as a measure of the lectin content of the samples. African yam bean was found to have the highest heamagglutinating activity. Tannins were found to be in low quantities. The presence of these anti-nutrients in relation to the nutritional value of the legume is discussed.
对在尼日利亚种植的六种未充分利用的豆类种子，即红腰豆和白腰豆、褐花和白花木豆、非洲豆薯和四棱豆，进行了不同抗营养因子的分析。通过毛细管气相色谱法鉴定出大豆皂醇B是利马豆和四棱豆中主要的皂醇。采用离子对高效液相色谱法分析了总肌醇磷酸酯和各肌醇磷酸酯（IP6、IP5、IP4和IP3）的含量，其含量与其他豆类种子的含量范围相当。在四棱豆中鉴定出痕量的羽扇豆碱为生物碱。所有豆类种子中均含有α-半乳糖苷，水苏糖是利马豆、非洲豆薯和四棱豆中的主要半乳糖苷，毛蕊花糖则存在于木豆中。通过估算血凝活性来测定样品中的凝集素含量。发现非洲豆薯的血凝活性最高。还发现单宁含量较低。本文讨论了这些抗营养物质与豆类营养价值之间的关系。
