Lee H S, Lee I S, Kang T C, Seo J, Lee B H
Department of Anatomy, College of Veterinary Medicine, Seoul National University, Suwon, Republic of Korea.
Anat Histol Embryol. 1998 Oct;27(5):331-3. doi: 10.1111/j.1439-0264.1998.tb00203.x.
In the present study, we performed the retrograde tracing method using the neurotropic pseudorabies virus bartha strain (PRV-Ba) and immunohistochemistry against CGRP, to identify whether CGRP exists in the gastric monosynaptic vagal circuit between dorsal motor nucleus of vagus nerve (DMV) and nucleus tractus solitarius (NTS). At the results, PRV immunoreactive neurons were found in both DMV and NTS. However, CGRP-immunoreactive cells were present only in NTS, which contained no double-labeled neurons for PRV and CGRP. These results suggest that CGRP may not have a neuronal function in gastric vagal circuit of rat.
在本研究中，我们使用嗜神经性伪狂犬病病毒巴塔株（PRV-Ba）进行逆行追踪法，并对降钙素基因相关肽（CGRP）进行免疫组织化学检测，以确定CGRP是否存在于迷走神经背核（DMV）和孤束核（NTS）之间的胃单突触迷走神经回路中。结果发现，在DMV和NTS中均发现了PRV免疫反应性神经元。然而，CGRP免疫反应性细胞仅存在于NTS中，其中没有PRV和CGRP的双标记神经元。这些结果表明，CGRP可能在大鼠胃迷走神经回路中不具有神经元功能。
