McBride W J, Chernet E, McKinzie D L, Lumeng L, Li T K
Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis 46202-4887, USA.
Alcohol. 1998 Nov;16(4):317-23. doi: 10.1016/s0741-8329(98)00021-4.
The densities of mu-opioid binding sites in the CNS of alcohol-naive adult male P and NP rats (N = 9 each line) were examined using quantitative autoradiography. Coronal sections (20 microm) were prepared from frozen brains and incubated in 5 nM [3H]DAMGO to label mu-opioid receptor sites. Nonspecific binding was determined in the presence of 1 microM DAMGO. The amount of [3H]DAMGO binding was (a) 20-25% higher in the olfactory tubercle, nucleus accumbens shell and core, and basolateral and lateral amygdaloid nuclei; (b) 15% higher in the lateral septal intermediate nucleus and caudate-putamen patches; and (c) 10-30% lower in the pyramidal and radiatum layers in the CA1 region of the anterior dorsal hippocampus, ventral dentate gyrus and CA1 pyramidal layer of the posterior hippocampus, and posterior medial cortical amygdaloid nucleus of the P compared to the NP rat. No line differences were found in any of the other regions examined (e.g., the cerebral cortical subregions and layers, thalamic nuclei, ventral tegmental area, ventral pallidum, lateral hypothalamus, other regions of the hippocampus, and several subcortical structures). The innate differences in the amount of binding to mu-opioid recognition sites in certain limbic structures, such as the nucleus accumbens, amygdala, and olfactory tubercle, of the P and NP lines may be factors contributing to their disparate alcohol drinking characteristics.
利用定量放射自显影技术检测了成年未接触酒精的雄性P系和NP系大鼠（每组9只）中枢神经系统中μ-阿片样物质结合位点的密度。从冰冻大脑制备冠状切片（20微米），并在5 nM [3H]DAMGO中孵育以标记μ-阿片样物质受体位点。在1 μM DAMGO存在的情况下测定非特异性结合。[3H]DAMGO的结合量在以下区域有差异：（a）嗅结节、伏隔核壳和核心以及基底外侧和外侧杏仁核中高20%-25%；（b）外侧隔中间核和尾壳核斑块中高15%；（c）与NP系大鼠相比，P系大鼠前背侧海马CA1区的锥体细胞层和辐射层、腹侧齿状回以及后海马CA1锥体细胞层和后内侧皮质杏仁核中低10%-30%。在其他检测区域（例如大脑皮质亚区域和层、丘脑核、腹侧被盖区、腹侧苍白球、外侧下丘脑、海马其他区域以及几个皮质下结构）未发现品系差异。P系和NP系大鼠在某些边缘结构（如伏隔核、杏仁核和嗅结节）中与μ-阿片样物质识别位点结合量的固有差异可能是导致它们饮酒特征不同的因素。
