Adcock D, Kressin D, Marlar R A
Department of Pathology, Colorado Kaiser Permanente Medical Group, University of Colorado Health Sciences Center, Denver, USA.
Blood Coagul Fibrinolysis. 1998 Sep;9(6):463-70. doi: 10.1097/00001721-199809000-00002.
This study evaluates the effects of time and temperature variables on routine coagulation assays [Prothrombin Time test and Activated Partial Thromboplastin Time (APTT) test]. Four different groups were studied: healthy volunteers, hospitalized patients not receiving anticoagulants, patients receiving oral anticoagulant therapy and patients receiving unfractionated heparin therapy. Samples were subjected to one of four conditions: (1) centrifuged immediately and stored at room temperature (20-22 degrees C); (2) centrifuged immediately and stored on ice (4 degrees C); (3) stored as whole blood without centrifugation, at room temperature and (4) stored without centrifugation, on ice. Coagulation tests were performed as soon as possible after phlebotomy and at specified times up to 24 h. Our data demonstrate that prothrombin time results are stable for up to 24 h, remaining constant regardless of storage conditions. APTT assays are stable for up to 8 h, except for patients receiving unfractionated heparin therapy. Heparinized samples, when stored uncentrifuged at room temperature, demonstrate a clinically significant shortening of the APTT and individual samples demonstrate a greater than 50% decrease in ex-vivo heparin levels at 4 h.
本研究评估时间和温度变量对常规凝血检测[凝血酶原时间试验和活化部分凝血活酶时间（APTT）试验]的影响。研究了四个不同的组：健康志愿者、未接受抗凝剂治疗的住院患者、接受口服抗凝治疗的患者以及接受普通肝素治疗的患者。样本被置于以下四种条件之一：（1）立即离心并在室温（20 - 22摄氏度）下储存；（2）立即离心并在冰上（4摄氏度）储存；（3）作为全血不离心，在室温下储存；（4）不离心，在冰上储存。采血后尽快并在长达24小时的特定时间进行凝血试验。我们的数据表明，凝血酶原时间结果在长达24小时内稳定，无论储存条件如何均保持不变。APTT检测在长达8小时内稳定，但接受普通肝素治疗的患者除外。肝素化样本在室温下不离心储存时，APTT会出现临床上显著的缩短，并且个别样本在4小时时体外肝素水平下降超过50%。
