Ustaçelebi S
Acta Virol. 1976 Aug;20(4):297-304.
Kinetics of interferon induction with wild type adenovirus 5, and three temperature-sensitive mutants, ts 1, ts 18 and ts 19 were examined in chick embryo cells (CEC) at permissive (31 degrees C) and non-permissive (38 degrees C) temperatures. Interferon levels reached a maximum four days after infection with the wild type and all ts mutants at 31 degrees C. However, at 38 degrees C, ts 18 and ts 19 failed to induce interferon, while wild type and ts 1 induced normal levels which reached a maximum by three days after infection. Striking differences in virion heat sensitivities between wild type, ts 1, ts 18 and ts 19 at both 50 degrees C and 52 degrees C were detected. These results suggested that ts 18 and ts 19 might each have a mutation in a gene coding for a virion structural component. The production of infectious progeny by shift-up experiments on Hela cells showed that mutant ts 18 was affected immediately after shift-up at all times throughout the growth cycle. On the other hand, interferon production in CEC by mutant ts 18 was only affected by shift-up during the first five hours of incubation, which indicated involvement of an early gene function in elliciting interferon production.
在允许温度（31摄氏度）和非允许温度（38摄氏度）下，研究了野生型腺病毒5以及三种温度敏感突变体ts 1、ts 18和ts 19在鸡胚细胞（CEC）中诱导干扰素的动力学。在31摄氏度时，野生型和所有ts突变体感染后四天，干扰素水平达到最大值。然而，在38摄氏度时，ts 18和ts 19未能诱导干扰素，而野生型和ts 1诱导的正常水平在感染后三天达到最大值。在50摄氏度和52摄氏度时，检测到野生型、ts 1、ts 18和ts 19之间病毒粒子热敏感性存在显著差异。这些结果表明，ts 18和ts 19可能各自在编码病毒粒子结构成分的基因中存在突变。通过在Hela细胞上进行升温实验产生感染性后代的情况表明，突变体ts 18在整个生长周期的任何时候升温后都立即受到影响。另一方面，突变体ts 18在CEC中产生干扰素仅在培养的前五个小时受到升温的影响，这表明早期基因功能参与了干扰素的产生。
