Taródi B
Institute of Microbiology, University Medical School of Szeged, Hungary.
Acta Virol. 1988 May;32(3):207-16.
Among several temperature sensitive mutants of human adenovirus type 12 (Ad12), H12ts15 was found to be defective in induction of interferon (IFN) in chick cells. This mutant was characterized as late mutant which synthesized defective III.a and penton-base antigens and failed to assemble into infectious particles at restrictive temperature. Moreover, its defect was manifested at permissive temperature, at which defective particles were readily formed. Temperature shift experiments revealed that an early event, but neither virus adsorption nor penetration, was blocked in the process of IFN induction. Therefore, it can be assumed that conformation of the adeno virion is important for IFN induction in chick cells. Conformation alteration caused by defective structural components of the capsid at restrictive temperature prevented IFN induction. This change, however, did not affect infectivity under similar conditions.
在人腺病毒12型（Ad12）的几种温度敏感突变体中，发现H12ts15在鸡细胞中诱导干扰素（IFN）方面存在缺陷。该突变体被表征为晚期突变体，它合成有缺陷的III.a和五聚体基底抗原，并且在限制温度下无法组装成感染性颗粒。此外，其缺陷在允许温度下也会表现出来，此时容易形成有缺陷的颗粒。温度转换实验表明，在IFN诱导过程中，一个早期事件被阻断，但病毒吸附和穿透均未受阻。因此，可以假定腺病毒粒子的构象对于鸡细胞中的IFN诱导很重要。在限制温度下，衣壳结构成分缺陷导致的构象改变阻止了IFN诱导。然而，这种变化在类似条件下并不影响感染性。
