Randall T A, Metzenberg R L
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA.
Mol Gen Genet. 1998 Oct;259(6):615-21. doi: 10.1007/s004380050855.
Complexity in and around the A and a mating type idiomorphs in Neurospora crassa was examined. Six sets of transcripts surrounding the idiomorphs were identified by Northern analysis. Several different patterns of regulation were observed. The two pairs of transcripts closest to the centromere-proximal idiomorph flanks (variable regions) exhibited mating type-specific size differences. DNA sequence was obtained for the region surrounding the four transcripts which showed mating type-specific size and expression. One of these pairs encoded amino acid sequences highly similar to a domain present in the plasma membrane ATPase. A mutant allele of one of these genes was induced by repeat-induced point mutation (RIP), which resulted in altered perithecial development and the elimination of ascospore production. These transcripts comprise a cluster of genes which may be involved in the control of mating and sexual development in N. crassa.
对粗糙脉孢菌A和a交配型特异序列及其周围的复杂性进行了研究。通过Northern分析鉴定出围绕特异序列的六组转录本。观察到几种不同的调控模式。最靠近着丝粒近端特异序列侧翼（可变区）的两对转录本表现出交配型特异性的大小差异。获得了显示交配型特异性大小和表达的四个转录本周围区域的DNA序列。其中一对编码的氨基酸序列与质膜ATPase中的一个结构域高度相似。这些基因中的一个通过重复诱导点突变（RIP）诱导产生了一个突变等位基因，导致子囊壳发育改变和子囊孢子产生的消除。这些转录本包含一组基因，可能参与粗糙脉孢菌交配和有性发育的控制。
