de la Rúa P, Serrano J, Galián J
Departamento de Biología Animal, Universidad de Murcia, Spain.
Mol Ecol. 1998 Nov;7(11):1543-7. doi: 10.1046/j.1365-294x.1998.00468.x.
The mitochondrial DNA (mtDNA) of individuals from 79 colonies of Apis mellifera from five Canary Islands was studied using the DraI test based on the restriction of PCR products of the tRNA(leu)-COII intergenic region. Five haplotypes of the African (A) lineage and one of the west European (C) lineage were found. The haplotypes A14 and A15 are described for the first time. These haplotypes have a new P sequence named P1. The wide distribution and high frequency of haplotype A15 suggest that it is characteristic of the Canarian Archipelago. Sources of haplotype variability of honeybee mtDNA in the Canary Islands (waves of colonization from Africa, queen importations, habitat diversification) are discussed.
利用基于tRNA(leu)-COII基因间隔区PCR产物限制性内切酶酶切的DraI检测法，对来自加那利群岛五个岛屿的79个意大利蜜蜂蜂群个体的线粒体DNA（mtDNA）进行了研究。发现了非洲（A）谱系的五种单倍型和西欧（C）谱系的一种单倍型。首次描述了单倍型A14和A15。这些单倍型具有一个名为P1的新P序列。单倍型A15的广泛分布和高频率表明它是加那利群岛的特征。讨论了加那利群岛蜜蜂mtDNA单倍型变异的来源（来自非洲的殖民浪潮、蜂王引进、栖息地多样化）。
