Schwaab V, Faure J, Dufaure J P, Drevet J R
Laboratoire de Biologie Cellulaire, UMR CNRS 6547-GEEM, Reproduction & Developpement Research Group, Université Blaise Pascal, Aubière, France.
Mol Reprod Dev. 1998 Dec;51(4):362-72. doi: 10.1002/(SICI)1098-2795(199812)51:4<362::AID-MRD2>3.0.CO;2-L.
We report here-using northern experiments, western blotting, and immunohistochemistry-on the findings that the plasma type glutathione peroxidase, GPx3, a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma, is also expressed at significant levels in tissues of the male genital tract including epididymis and vas deferens. Within the epididymis and the kidney, the accumulation of the GPx3 mRNA and protein were investigated during postnatal development and found to be temporally regulated in a tissue-specific manner. Furthermore, we show here that androgen withdrawal by castration down regulates the expression of the GPx3 gene both in the epididymis and vas deferens while GPx3 expression in the kidney was found to be androgen-independent. Finally, immunohistochemistry data reveals that within the epididymis GPx3 distribution is quite peculiar suggesting the existence in this organ of complex traductional and/or transcriptional regulatory processes.
我们在此报告——通过Northern实验、蛋白质印迹法和免疫组织化学——发现血浆型谷胱甘肽过氧化物酶GPx3是血浆中还原脂质氢过氧化物和过氧化氢的主要酶，在男性生殖道组织包括附睾和输精管中也有显著表达。在附睾和肾脏中，研究了出生后发育过程中GPx3 mRNA和蛋白质的积累情况，发现其在不同组织中呈现出时间特异性调控。此外，我们在此表明，去势导致雄激素撤退会下调附睾和输精管中GPx3基因的表达，而肾脏中GPx3的表达则不受雄激素影响。最后，免疫组织化学数据显示，在附睾内GPx3的分布颇为奇特，这表明该器官中存在复杂的翻译和/或转录调控过程。
