Ping X, Akeda Y, Fujita K I, Tanaka T, Taniguchi M
Faculty of Science, Osaka City University, Osaka, Japan.
J Antibiot (Tokyo). 1998 Sep;51(9):852-6. doi: 10.7164/antibiotics.51.852.
The mechanism of the antifungal action of AKD-2C was studied by using Torulaspora delbrueckii IFO 1621 as a model. AKD-2C slightly inhibited the incorporation of radioactive precursors into protein, RNA and lipid, but not into DNA. On the other hand, AKD-2C greatly enhanced the leakage of K+ ions from treated cells and showed a potent effect on liposomal glucose leakage. Using electron microscopic studies, though drastic morphological changes were not observed, an increase in cell membrane irregularities and swelling of the mitochondria caused by AKD-2C were demonstrated. These results suggest that the antifungal action of AKD-2C is due to effects on the yeast cell membrane.
以戴尔布有孢圆酵母IFO 1621为模型研究了AKD-2C的抗真菌作用机制。AKD-2C轻微抑制放射性前体掺入蛋白质、RNA和脂质，但不抑制掺入DNA。另一方面，AKD-2C极大地增强了处理细胞中K+离子的泄漏，并对脂质体葡萄糖泄漏显示出强效作用。通过电子显微镜研究，虽然未观察到剧烈的形态变化，但证实了AKD-2C导致细胞膜不规则性增加和线粒体肿胀。这些结果表明，AKD-2C的抗真菌作用归因于对酵母细胞膜的影响。
