Mögel M, Krüger E, Krug H F, Seidel A
Forschungszentrum Karlsruhe, Institute of Toxicology, Germany.
Toxicol Lett. 1998 Aug;96-97:25-32. doi: 10.1016/s0378-4274(98)00046-0.
In order to study the inflammatory potential of ozone on the airway tissue, we developed an in vitro model system in which human bronchial epithelial cells (BEAS 2B) and human umbilical vein endothelial cells (ECV304) were able to communicate with each other. The BEAS 2B cells were grown on filter supports which were inserted into six-well culture dishes. Endothelial cells were cultivated on the bottom of the basolateral compartment. The upper epithelial cells were exposed to 0.15 ppm ozone for 90 min. Supernatants were collected after 1, 4 and 24 h and were quantified for IL-6 and IL-8 secretion. At the same time points we measured the expression of ICAM-1 on the umbilical vein endothelial cells. Exposure of the coculture-system to air or ozone induced the production of IL-6 as well as IL-8 that exceeded the sum of the amounts produced by the two cell types when exposed separately. At 24 h after ozone exposure the IL-6 and IL-8 levels were significantly elevated compared with the air treated cells. Concerning the ICAM-1 expression on ECV304 cells we found elevated ICAM-1 levels on cells which had been cocultured with BEAS 2B cells compared with cells cultured alone. This might be a hint for the secretion of a soluble factor that acts as a mediator in amplifying the response of epithelial cells.
为了研究臭氧对气道组织的炎症作用，我们建立了一种体外模型系统，其中人支气管上皮细胞（BEAS 2B）和人脐静脉内皮细胞（ECV304）能够相互交流。BEAS 2B细胞生长在插入六孔培养皿的滤膜支架上。内皮细胞培养在基底外侧腔室的底部。上层上皮细胞暴露于0.15 ppm臭氧中90分钟。在1、4和24小时后收集上清液，并对IL-6和IL-8分泌进行定量。在相同时间点，我们测量脐静脉内皮细胞上ICAM-1的表达。将共培养系统暴露于空气或臭氧中会诱导IL-6和IL-8的产生，其产量超过两种细胞类型单独暴露时产生的量之和。在臭氧暴露24小时后，与空气处理的细胞相比，IL-6和IL-8水平显著升高。关于ECV304细胞上ICAM-1的表达，我们发现与单独培养的细胞相比，与BEAS 2B细胞共培养的细胞上ICAM-1水平升高。这可能暗示一种可溶性因子的分泌，该因子作为一种介质在放大上皮细胞的反应中起作用。
