Alink G M, Sjögren M, Bos R P, Doekes G, Kromhout H, Scheepers P T
Department of Toxicology, Agricultural University, Wageningen, The Netherlands.
Toxicol Lett. 1998 Aug;96-97:209-13. doi: 10.1016/s0378-4274(98)00074-5.
The effect of airborne particles from diesel exhaust, rubber and metal industry, urban air and biological sources (poultry, pig farming, compost industry) on gap-junctional intercellular communication (GJIC) were compared, using HEPA1c1c7 cells. Particles as such were compared with aqueous and organic extracts. Significant inhibition of GJIC by particle suspensions was only observed for the diesel and rubber samples, and for one biological sample (compost). Up to 83% of the inhibition of the whole suspension could be attributed to the particles as such. Washing the particles with organic solvents (aceton, methanol, hexane) did not result in a significant loss of activity from the particles, although the organic fractions showed a significant activity towards GJIC. More active organics was eluted from the rubber industry particles than from the diesel particles by the organic solvent. It is suggested that cancer promoting potential as measured by inhibition of GJIC may vary widely depending on the particle source, and that this effect may be exerted by the particles as such and/or by means of tightly bound bio-active material to the surface.
使用HEPA1c1c7细胞比较了来自柴油尾气、橡胶和金属工业、城市空气以及生物源（家禽养殖、养猪业、堆肥行业）的空气传播颗粒对间隙连接细胞间通讯（GJIC）的影响。将此类颗粒与水提取物和有机提取物进行了比较。仅在柴油和橡胶样品以及一个生物样品（堆肥）中观察到颗粒悬浮液对GJIC有显著抑制作用。整个悬浮液高达83%的抑制作用可归因于颗粒本身。用有机溶剂（丙酮、甲醇、己烷）洗涤颗粒后，颗粒活性没有显著损失，尽管有机部分对GJIC显示出显著活性。有机溶剂从橡胶工业颗粒中洗脱的活性有机物比从柴油颗粒中洗脱的更多。研究表明，通过抑制GJIC测量的致癌潜力可能因颗粒来源而异，并且这种影响可能由颗粒本身和/或通过与颗粒表面紧密结合的生物活性物质来施加。
