Hill E M, Eling T, Nettesheim P
Laboratory of Molecular Carcinogenesis, NIEHS, Research Triangle Park, NC, USA.
Toxicol Lett. 1998 Aug;96-97:239-44. doi: 10.1016/s0378-4274(98)00078-2.
The expression of 15-lipoxygenase (15-LO) and prostaglandin H synthase-2 (PGHS-2) was studied in retinoic acid (RA) sufficient and RA-deficient normal human tracheobronchial epithelial (NHTBE) cell cultures. In the absence of RA, in which the cultures became squamous metaplastic, neither 15-LO nor PGHS-2 were expressed. In RA-sufficient cultures, which differentiated into a mucociliary epithelium, both enzymes were expressed: PGHS-2 during early phases and 15-LO during late stages of differentiation. Depending on the stage of differentiation, the RA-sufficient cultures produced PGE, and in the presence of exogenous linoleic acid (LA) 13-HODE. Experiments are underway to examine the effects of inflammatory cytokines on eicosanoid metabolism and the role these metabolites play in airway diseases.
在视黄酸（RA）充足和RA缺乏的正常人气管支气管上皮（NHTBE）细胞培养物中研究了15-脂氧合酶（15-LO）和前列腺素H合酶-2（PGHS-2）的表达。在没有RA的情况下，培养物发生鳞状化生，15-LO和PGHS-2均未表达。在分化为黏液纤毛上皮的RA充足的培养物中，两种酶均有表达：PGHS-2在分化早期表达，15-LO在分化后期表达。根据分化阶段，RA充足的培养物产生前列腺素E（PGE），在外源亚油酸（LA）存在的情况下产生13-羟基十八碳二烯酸（13-HODE）。正在进行实验以研究炎性细胞因子对类花生酸代谢的影响以及这些代谢产物在气道疾病中的作用。
