Maxwell M, Galanopoulos T, Neville-Golden J, Hedley-Whyte E T, Antoniades H N
Neurosurgical Service, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA.
Neuropathol Appl Neurobiol. 1998 Oct;24(5):337-45. doi: 10.1046/j.1365-2990.1998.00138.x.
Platelet-derived growth factor (PDGF) has been implicated in the processes regulating gliogenesis in the CNS. Conflicting in vivo data in rodents have variously implicated either glia or neurons as being the primary source of PDGF. We have used in situ hybridization and immunocytochemical analysis to study the in vivo expression and cellular localization of PDGF-A, sis/PDGF-B, together with the two PDGF receptors alpha and beta, in developing human forebrain. In this study we demonstrate the strong expression of mRNA and protein of both PDGF chains, A and B, and their receptors, alpha and beta, in human embryonic glial cells. The neurons, in contrast to glial cells, expressed lower levels of PDGF and PDGF-receptor mRNAs and protein. Identification of the cell types expressing the PDGF and PDGF-receptor mRNAs was achieved by counterstaining with antibodies specific for glial cells (GFAP) and neurons (NF). The predominant glial-specific expression of both PDGF-A and PDGF-B, together with the coexpression of their receptors alpha and beta, suggests an important role for the PDGF isoforms in the development of human embryonic glial cells and neurons in vivo.
血小板衍生生长因子（PDGF）与中枢神经系统中调节神经胶质生成的过程有关。啮齿动物体内相互矛盾的数据表明，神经胶质细胞或神经元是PDGF的主要来源。我们使用原位杂交和免疫细胞化学分析方法，研究了PDGF-A、sis/PDGF-B以及两种PDGF受体α和β在发育中的人类前脑的体内表达和细胞定位。在本研究中，我们证明了PDGF的A链和B链及其受体α和β的mRNA和蛋白质在人类胚胎神经胶质细胞中强烈表达。与神经胶质细胞相比，神经元中PDGF和PDGF受体的mRNA及蛋白质表达水平较低。通过用针对神经胶质细胞（GFAP）和神经元（NF）的特异性抗体进行复染，确定了表达PDGF和PDGF受体mRNA的细胞类型。PDGF-A和PDGF-B在神经胶质细胞中的主要特异性表达，以及它们的受体α和β的共表达，表明PDGF异构体在人类胚胎神经胶质细胞和神经元的体内发育中起重要作用。
