Jermutus L, Ryabova L A, Plückthun A
Biochemisches Institut, Universität Zürich, Switzerland.
Curr Opin Biotechnol. 1998 Oct;9(5):534-48. doi: 10.1016/s0958-1669(98)80042-6.
Prokaryotic and eukaryotic in vitro translation systems have recently become the focus of increasing interest for tackling fundamental problems in biochemistry. Cell-free systems can now be used to study the in vitro assembly of membrane proteins and viral particles, rapidly produce and analyze protein mutants, and enlarge the genetic code by incorporating unnatural amino acids. Using in vitro translation systems, display techniques of great potential have been developed for protein selection and evolution. Furthermore, progress has been made to efficiently produce proteins in batch or continuous cell-free translation systems and to elucidate the molecular causes of low yield and find possible solutions for this problem.
原核生物和真核生物的体外翻译系统最近已成为解决生物化学基本问题的越来越受关注的焦点。无细胞系统现在可用于研究膜蛋白和病毒颗粒的体外组装，快速产生和分析蛋白质突变体，以及通过掺入非天然氨基酸来扩展遗传密码。利用体外翻译系统，已开发出具有巨大潜力的展示技术用于蛋白质筛选和进化。此外，在批量或连续无细胞翻译系统中高效生产蛋白质以及阐明产量低的分子原因并找到解决该问题的可能方案方面也取得了进展。
