Stano J, Nemec P, Bezáková L, Kákoniová D, Kovács P, Neubert K, Liskova D, Andriamainty F, Micieta K
Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
Acta Biochim Pol. 1998;45(2):621-6.
Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.
小黄瓜（Cucumis sativus L.）的细胞悬浮液用吐温-80进行透化处理，并用戊二醛固定。β-半乳糖苷酶的最适pH为4.9，最适温度为58℃。该酶催化的水解反应在3小时内呈线性，底物转化率为60-68%。具有高β-半乳糖苷酶活性且在长期储存中稳定的细胞表现出有价值的技术特性。
