Saika S, Kawashima Y, Miyamoto T, Okada Y, Tanaka S, Yamanaka O, Ohnishi Y, Ooshima A, Yamanaka A
Department of Ophthalmology, Wakayama Medical College, Japan.
Jpn J Ophthalmol. 1998 Sep-Oct;42(5):368-72. doi: 10.1016/s0021-5155(98)00033-1.
Lens capsules become fibrotic after cataract extraction. A variety of extracellular matrix (ECM) components accumulate on these capsules in association with the proliferation of lens epithelial cells. To provide a better understanding of the process of capsular fibrosis, we assessed the types of proteoglycans (PG) in human lens capsules with intraocular lenses (IOL). Lens capsules containing IOLs were removed from 1 patient with proliferative vitreoretinopathy and 1 patient with proliferative diabetic retinopathy. After treatment with chondroitinase ABC, tissue sections were processed for immunohistochemical detection of the proteoglycans including chondroitin, large PG, chondroitin 4-sulfate PG, chondroitin 6-sulfate PG, dermatan sulfate PG, and keratan sulfate PG. Extracellular matrix was found on the inner surface of the capsular bag. In association with what appeared to be proliferating lens epithelial cells, each of the six types of PG was present in the ECM on the capsules. All six types of PG might be involved in the fibrosis and opacification of lens capsules after extraction of the cataract and implantation of the IOL.
白内障摘除术后晶状体囊膜会发生纤维化。多种细胞外基质（ECM）成分会随着晶状体上皮细胞的增殖而在这些囊膜上积聚。为了更好地理解囊膜纤维化的过程，我们评估了植入人工晶状体（IOL）的人晶状体囊膜中蛋白聚糖（PG）的类型。从1例增殖性玻璃体视网膜病变患者和1例增殖性糖尿病视网膜病变患者中取出含有IOL的晶状体囊膜。用硫酸软骨素酶ABC处理后，对组织切片进行免疫组织化学检测，以检测包括硫酸软骨素、大分子PG、硫酸软骨素4-硫酸酯PG、硫酸软骨素6-硫酸酯PG、硫酸皮肤素PG和硫酸角质素PG在内的蛋白聚糖。在囊袋的内表面发现了细胞外基质。与似乎正在增殖的晶状体上皮细胞相关，六种类型的PG中的每一种都存在于囊膜上的ECM中。所有六种类型的PG可能都参与了白内障摘除和IOL植入后晶状体囊膜的纤维化和浑浊形成。
