Mebius R E, Schadee-Eestermans I L, Weissman I L
Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Cell Adhes Commun. 1998;6(2-3):97-103. doi: 10.3109/15419069809004464.
During fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules briefly express the Peyer's patch addressin MAdCAM-1. This allows initial seeding by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta 7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. It was found that the CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1. They can differentiate into natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. In addition to LN, CD4+CD3- cells can also be found in fetal spleen starting at 13.5 dpc, while absent from fetal liver. In view of the necessity of lymphotoxin in lymphoid organ development, it is thought that the novel subset of CD4+CD3- LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.
在小鼠胎儿淋巴结器官发生过程中，淋巴结毛细血管后高内皮微静脉短暂表达派尔集合淋巴结地址素MAdCAM-1。这使得两种异常淋巴细胞群体能够进行初始定植，这两种群体选择性表达派尔集合淋巴结归巢受体整合素α4β7：CD4⁺CD3⁻寡谱系祖细胞和TCRγδ⁺T细胞。研究发现，CD4⁺CD3⁻细胞是谱系受限的祖细胞，表达表面淋巴毒素-β（LTβ）和趋化因子受体BLR1。它们可分化为自然杀伤细胞、树突状抗原呈递细胞以及结局未知的滤泡细胞，但这些细胞不会成为T或B淋巴细胞。除淋巴结外，在胚胎第13.5天开始，胎儿脾脏中也可发现CD4⁺CD3⁻细胞，而胎儿肝脏中则没有。鉴于淋巴毒素在淋巴器官发育中的必要性，人们认为CD4⁺CD3⁻LTβ⁺胎儿细胞的新亚群在淋巴组织结构发育中起重要作用。
