伴有和不伴有肝硬化的酗酒患者中庚型肝炎病毒感染的患病率。

Prevalence of hepatitis G-virus infection in alcohol abusing patients with and without liver cirrhosis.

作者信息

Schöniger-Hekele M, Petermann D, Lesch O M, Müller C

机构信息

Klinische Abteilung Gastroenterologie und Hepatologie, Universitätsklinik für Innere Medizin IV, Vienna, Austria.

出版信息

Wien Klin Wochenschr. 1998 Oct 16;110(19):686-90.

PMID:9823622

Abstract

BACKGROUND

Hepatitis G (GBV-C/HGV) is an RNA-virus belonging to the flavivirus family and is capable of inducing hepatitis in rare cases. Its importance as a co-factor in the pathogenesis of liver disease needs to be clarified.

AIMS

To determine the prevalence of HGV in chronic alcoholics with and without liver cirrhosis.

PATIENTS

86 alcoholics, 44 with liver cirrhosis and 42 without liver cirrhosis, were investigated; 93 healthy individuals served as controls.

METHODS

Serum was tested for GBV-C/HGV-RNA by reverse-transcription polymerase chain reaction (RT-PCR) and for anti-E2, a marker of resolved GBV-C/HGV infection, by ELISA. GBV-C/HGV-RNA positive samples were sequenced and the GBV-C/HGV subtype determined.

RESULTS

Eight out of 86 (9.3%) alcoholics were GBV-C/HGV-RNA positive, as compared to 2 out of 93 (2.2%) healthy controls (n. s.). Twenty-one (24.4%) alcoholics had anti-E2 in serum, whereas this antibody was found in 12 (12.9%) healthy persons only (n. s.). However, significantly more alcoholic patients (33.7%) than healthy controls (15.1%) had past or present contact with GBV-C/HGV (p = 0.006). 11.4% of alcoholic patients with liver cirrhosis and 7.1% of alcoholic patients without liver cirrhosis showed GBV-C/HGV-RNA. 34.1% of alcoholic patients with liver cirrhosis and 16.6% of alcoholic patients without liver cirrhosis had anti-E2. Among the 44 patients with liver cirrhosis, 8 out of 11 (72.7%) patients with variceal bleeding, but only 11 of 33 patients without bleeding had contact with GBV-C/HGV (p = 0.05). Seven out of 8 GBV-C/HGV-RNA positive alcoholics had genotype 2a, 1 had type 1a of GBV-C/HGV.

CONCLUSION

Alcoholic patients have a significantly higher contact rate with GBV-C/HGV as compared to healthy controls. Alcoholics with liver cirrhosis tend to be more frequently infected than alcoholic patients without liver cirrhosis. A previous variceal bleeding episode is significantly associated with GBV-C/HGV infection.

摘要

背景

庚型肝炎病毒（GBV-C/HGV）是一种属于黄病毒科的RNA病毒，在罕见情况下可引发肝炎。其作为肝病发病机制中的一个辅助因素的重要性有待阐明。

目的

确定肝硬化和非肝硬化慢性酗酒者中庚型肝炎病毒（HGV）的感染率。

患者

对86名酗酒者进行了调查，其中44名患有肝硬化，42名未患肝硬化；93名健康个体作为对照。

方法

采用逆转录聚合酶链反应（RT-PCR）检测血清中的GBV-C/HGV-RNA，采用酶联免疫吸附测定（ELISA）检测抗-E2，抗-E2是已清除的GBV-C/HGV感染的标志物。对GBV-C/HGV-RNA阳性样本进行测序并确定GBV-C/HGV亚型。

结果

86名酗酒者中有8名（9.3%）GBV-C/HGV-RNA呈阳性，而93名健康对照中有2名（2.2%）呈阳性（无统计学差异）。21名（24.4%）酗酒者血清中有抗-E2，而仅12名（12.9%）健康人有此抗体（无统计学差异）。然而，有过或正接触GBV-C/HGV的酗酒患者（33.7%）明显多于健康对照（15.1%）（p = 0.006）。11.4%的肝硬化酗酒患者和7.1%的非肝硬化酗酒患者GBV-C/HGV-RNA呈阳性。34.1%的肝硬化酗酒患者和16.6%的非肝硬化酗酒患者有抗-E2。在44名肝硬化患者中，11名静脉曲张出血患者中有8名（72.7%）接触过GBV-C/HGV，但33名未出血患者中只有11名接触过（p = 0.05）。8名GBV-C/HGV-RNA阳性酗酒者中有7名基因型为2a，1名基因型为GBV-C/HGV 1a型。