Palmen M J, Dieleman L A, Soesatyo M, Peña A S, Meuwissen S G, van Rees E P
Department of Cell Biology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Dig Dis Sci. 1998 Nov;43(11):2518-25. doi: 10.1023/a:1026606904531.
Glucocorticosteroids (GCS) are effective in treatment of inflammatory bowel disease (IBD), but also have unwanted systemic side effects. Here, we describe the effects of budesonide and dexamethasone on acute experimental colitis and on T cells in thymus and spleen, as well as the effect of budesonide treatment on relapsing colitis. Acute colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) in ethanol, and a relapse was induced by an intraperitoneal booster of TNBS. GCS were administered intrarectally on days 1, 4, and 6 after induction of acute colitis or a relapse. Inflammatory cells in the colon were studied on day 7, and in acute colitis also on days 13 and 16. Budesonide treatment in acute and relapsing colitis resulted in reduction of macroscopic damage and decreased the numbers of macrophages and neutrophils in the colon. Dexamethasone was less effective. Dexamethasone, but not budesonide, reduced the number of T cells in the thymus. It is concluded that local budesonide is more effective in treatment of acute experimental colitis than dexamethasone and, in contrast to dexamethasone, did not cause a general suppression of T cells. Although budesonide was very effective in the treatment of relapsing colitis, this effect was not accomplished by affecting the number of T cells in the colon.
糖皮质激素（GCS）对炎症性肠病（IBD）有效，但也有不良的全身副作用。在此，我们描述了布地奈德和地塞米松对急性实验性结肠炎以及胸腺和脾脏中T细胞的影响，以及布地奈德治疗对复发性结肠炎的影响。急性结肠炎通过在乙醇中结肠内给予2,4,6-三硝基苯磺酸（TNBS）诱导，复发通过TNBS腹腔内加强注射诱导。在急性结肠炎或复发诱导后的第1、4和6天经直肠给予GCS。在第7天研究结肠中的炎症细胞，在急性结肠炎中还在第13和16天进行研究。布地奈德治疗急性和复发性结肠炎可减少宏观损伤，并减少结肠中巨噬细胞和中性粒细胞的数量。地塞米松效果较差。地塞米松而非布地奈德可减少胸腺中T细胞的数量。得出的结论是，局部布地奈德在治疗急性实验性结肠炎方面比地塞米松更有效，并且与地塞米松不同，不会导致T细胞的普遍抑制。尽管布地奈德在治疗复发性结肠炎方面非常有效，但这种效果并非通过影响结肠中T细胞的数量来实现。
