Rea G, Laurenzi M, Tranquilli E, D'Ovidio R, Federico R, Angelini R
Dipartimento di Biologia, Università Roma Tre, Rome, Italy.
FEBS Lett. 1998 Oct 23;437(3):177-82. doi: 10.1016/s0014-5793(98)01219-8.
A chickpea cDNA encoding a cell wall copper amine oxidase (CuAO) was cloned and characterised. The 2010 bp open reading frame encodes a protein of 76.5 kDa which shares significant primary structure homology with other known CuAOs. Southern blot analysis indicates that in chickpea CuAO is encoded by a single gene or a small gene family. This cDNA was essential for studying the role of CuAO during seedling development and wound healing in chickpea seedlings. CuAO transcript level and activity were modulated during seedling development in parallel with cell maturation. Moreover, mechanical wounding induced a rapid increase of CuAO mRNA accumulation and enzyme activity which remained high during the wound-healing process. Aminoguanidine, a specific CuAO inhibitor, decreased the deposition of lignin-suberin barrier along the lesion. CuAO may be a limiting factor in H2O2 production in the cell wall of chickpea seedlings and its expression seems to integrate with the remodelling of plant cell wall occurring during ontogenesis and wound healing.
克隆并鉴定了一个编码细胞壁铜胺氧化酶（CuAO）的鹰嘴豆cDNA。2010 bp的开放阅读框编码一个76.5 kDa的蛋白质，该蛋白质与其他已知的CuAO具有显著的一级结构同源性。Southern杂交分析表明，在鹰嘴豆中CuAO由一个单基因或一个小基因家族编码。该cDNA对于研究CuAO在鹰嘴豆幼苗发育和伤口愈合过程中的作用至关重要。在幼苗发育过程中，CuAO转录水平和活性与细胞成熟平行调节。此外，机械损伤诱导CuAO mRNA积累和酶活性迅速增加，在伤口愈合过程中保持高水平。氨基胍是一种特异性CuAO抑制剂，它减少了木质素-栓质屏障沿损伤部位的沉积。CuAO可能是鹰嘴豆幼苗细胞壁中过氧化氢产生的限制因素，其表达似乎与个体发育和伤口愈合过程中发生的植物细胞壁重塑整合在一起。
