TT病毒（TTV）的新基因型及基于限制性片段长度多态性的基因分型检测方法

New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism.

作者信息

Tanaka Y, Mizokami M, Orito E, Ohno T, Nakano T, Kato T, Kato H, Mukaide M, Park Y M, Kim B S, Ueda R

机构信息

Second Department of Medicine, Nagoya City University Medical School, Mizuho, Japan.

出版信息

FEBS Lett. 1998 Oct 23;437(3):201-6. doi: 10.1016/s0014-5793(98)01231-9.

DOI:10.1016/s0014-5793(98)01231-9

PMID:9824290

Abstract

A phylogenetic analysis, using the open reading frame I sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that the virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability of the six clusters was confirmed by bootstrap analysis. On the basis of these sequence data, a new simple genotyping assay based on a restriction fragment length polymorphism of TTV was developed. Using the enzymes NdeI and PstI, followed by cleavage with NlaIII or MseI, it was possible to distinguish between the six TTV genotypes. This system will provide the framework for future detailed epidemiological and clinical investigations.

摘要

一项系统发育分析使用了从不同地理区域获得的93种TT病毒（TTV）的开放阅读框I序列，结果表明该病毒可分为六种不同的基因型，包括三种迄今未报道的基因型。自展分析证实了这六个聚类的高可靠性。基于这些序列数据，开发了一种基于TTV限制性片段长度多态性的新的简单基因分型检测方法。使用NdeI和PstI酶，随后用NlaIII或MseI切割，可以区分六种TTV基因型。该系统将为未来详细的流行病学和临床研究提供框架。