Feng M, Chou D, Liaw Y, Lai M
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Immunology. 1998 Oct;95(2):185-92. doi: 10.1046/j.1365-2567.1998.00589.x.
T-cell receptor (TCR) interacts with an antigenic peptide deeply buried in the major histocompatibility complex (MHC) molecule. How class II MHC is contacted by TCR during antigen recognition remains largely elusive. Here we used a panel of I-Ek mutants to identify two I-Ek residues that were frequently contacted by TCR among a large pool of T cells specific for the same antigen. The restricted TCR interaction with I-Ek was independent of the antigen peptides. We also identified a dominant heteroclitic residue on I-Ek, beta81H, in which mutation led to increased recognition of antigens in individual T-cell clones. Moreover, both the conserved TCR-I-Ek interaction and the heteroclitic TCR-I-Ek recognition were detected in T lymphocytes freshly isolated from mice primed with the specific antigens. The identical TCR-I-Ek interaction in a heterogeneous T-cell population suggested the dominance of invariant TCR-class II MHC interaction.
T细胞受体（TCR）与深埋于主要组织相容性复合体（MHC）分子中的抗原肽相互作用。在抗原识别过程中，TCR如何与II类MHC接触在很大程度上仍不清楚。在这里，我们使用一组I-Ek突变体，在针对同一抗原的大量T细胞中，鉴定出两个经常被TCR接触的I-Ek残基。TCR与I-Ek的受限相互作用独立于抗原肽。我们还在I-Ek上鉴定出一个显性异嗜性残基β81H，其突变导致单个T细胞克隆对抗原的识别增加。此外，在从用特定抗原免疫的小鼠中新鲜分离的T淋巴细胞中，检测到保守的TCR-I-Ek相互作用和异嗜性TCR-I-Ek识别。异质性T细胞群体中相同的TCR-I-Ek相互作用表明不变的TCR-II类MHC相互作用占主导地位。
