Hirschler J, Halgand F, Forest E, Fontecilla-Camps J C
Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel (CEA-CNRS) 41, Grenoble, France.
Protein Sci. 1998 Jan;7(1):185-92. doi: 10.1002/pro.5560070119.
The inclusion of protein contaminants into crystals of turkey egg white lysozyme (TEWL) was investigated by electrospray mass spectrometry of the dissolved crystals. The results show that significant amounts of the structurally related contaminant hen egg white lysozyme (HEWL) are included in the crystals of TEWL. The structurally unrelated contaminant RNAse A, on the other hand, is not included. The X-ray diffraction data statistics of a hybrid TEWL/HEWL crystal and an uncontaminated TEWL crystal were of similar quality. This indicates that, even though the crystals contain much higher levels of the contaminant than one would have expected after a recrystallization experiment, they are still suitable for X-ray diffraction experiments. However, attempts to detect the presence of the contaminant in the crystal by crystallographic structure refinement did not yield conclusive results.
通过对溶解晶体进行电喷雾质谱分析，研究了火鸡卵清溶菌酶（TEWL）晶体中蛋白质污染物的掺入情况。结果表明，大量结构相关的污染物鸡卵清溶菌酶（HEWL）掺入到了TEWL晶体中。另一方面，结构不相关的污染物核糖核酸酶A并未掺入。混合的TEWL/HEWL晶体和未受污染的TEWL晶体的X射线衍射数据统计质量相似。这表明，尽管晶体中污染物的含量比重结晶实验后预期的要高得多，但它们仍适用于X射线衍射实验。然而，通过晶体结构精修检测晶体中污染物存在的尝试并未得出确凿结果。
