Troncone R, Mazzarella G, Leone N, Mayer M, De Vincenzi M, Greco L, Auricchio S
Department of Pediatrics, University Federico II, Naples, Italy.
Gut. 1998 Oct;43(4):484-9. doi: 10.1136/gut.43.4.484.
CD3 and gamma delta cells in the rectal mucosa increase after local instillation of gluten in children with coeliac disease and in half of their siblings. Aim- To establish an in vitro system for assessing immunological changes induced by gluten in the rectum.
Rectal biopsy specimens obtained from 13 treated coeliac children, nine of their siblings, and nine controls were cultured in vitro with a peptic-tryptic digest of gliadin or ovalbumin. CD3 and CD25 cells were counted, and the expression of adhesion molecules evaluated.
In the lamina propria of coeliac biopsy samples cultured with gliadin, but not in those from controls, the expression of vascular cell adhesion molecule 1 (VCAM-1) was enhanced, and the number of CD25 cells was significantly higher than in those cultured in medium alone; the density of intraepithelial CD3 cells was also significantly higher. No differences were noted in coeliac biopsy specimens cultured with ovalbumin. A discriminant analysis allowed correct classification of all controls and all coeliacs but one, but three of nine siblings were allocated to the coeliac group.
Our data confirm that gliadin is able to activate cell mediated immunity in the rectal mucosa in coeliac patients and in a subset of their first degree relatives.
腹腔疾病患儿及其半数同胞在直肠黏膜局部滴注麸质后，直肠黏膜中的CD3细胞和γδ细胞会增加。目的：建立一种体外系统，以评估麸质在直肠中诱导的免疫变化。
从13名接受治疗的腹腔疾病患儿、他们的9名同胞以及9名对照者获取直肠活检标本，将其与麦醇溶蛋白或卵清蛋白的胃蛋白酶 - 胰蛋白酶消化物进行体外培养。对CD3和CD25细胞进行计数，并评估黏附分子的表达。
在用麦醇溶蛋白培养的腹腔疾病活检样本的固有层中，血管细胞黏附分子1（VCAM - 1）的表达增强，而在对照样本中未增强，并且CD25细胞的数量显著高于仅在培养基中培养的样本；上皮内CD3细胞的密度也显著更高。在用卵清蛋白培养的腹腔疾病活检标本中未观察到差异。判别分析能够正确分类所有对照者和除一名之外的所有腹腔疾病患者，但9名同胞中有3名被归入腹腔疾病组。
我们的数据证实，麦醇溶蛋白能够激活腹腔疾病患者及其一部分一级亲属直肠黏膜中的细胞介导免疫。
