Johnson B K, Varma M G
Trans R Soc Trop Med Hyg. 1976;70(3):230-4. doi: 10.1016/0035-9203(76)90045-6.
Aedes aegypti cells exposed to infectious complexes of WN or YF virus and homologous antiserum produced lower yields of virus over a 10-day observation period than were produced by Aedes aegypti cells treated with a comparable dose of virus mixed with non-immune serum. When Ae. aegypti cells were infected with WN virus mixed with MVE, NTA, DEN-2 or YF antisera the virus yield over 10 days was lower than in cell cultures infected at similar titres with mixtures of WN virus with non-immune serum. If Ae. aegypti cell cultures were infected with mixtures of YF virus and WN or DEN-2 antiserum the resulting production of virus was lower over 10 days than in virus mixed with non-immune serum. Human serum samples from the field were tested for the presence of antibody by preincubation of the serum with WN virus prior to inoculation on to mosquito cell cultures. The results indicated that this method is as sensitive in detecting antibody as a mouse neutralization test using regression analysis of average survival time.
在10天的观察期内，暴露于西尼罗病毒或黄热病病毒感染复合物及同源抗血清的埃及伊蚊细胞所产生的病毒产量，低于用等量病毒与非免疫血清混合处理的埃及伊蚊细胞所产生的病毒产量。当埃及伊蚊细胞被感染了与墨累山谷脑炎病毒、日本乙型脑炎病毒、登革2型病毒或黄热病病毒抗血清混合的西尼罗病毒时，10天内的病毒产量低于用西尼罗病毒与非免疫血清混合物以相似滴度感染的细胞培养物。如果埃及伊蚊细胞培养物被感染了黄热病病毒与西尼罗病毒或登革2型病毒抗血清的混合物，那么在10天内产生的病毒量低于与非免疫血清混合的病毒。对接种到蚊细胞培养物之前预先与西尼罗病毒孵育的现场采集的人血清样本进行抗体检测。结果表明，该方法在检测抗体方面与使用平均存活时间回归分析的小鼠中和试验一样灵敏。
