Roland I H, Bougelet C, Ninane N, Arnould T, Michiels C, Remacle J
Laboratoire de Biochimie et Biologie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Cardiovasc Drugs Ther. 1998 Sep;12(4):375-81. doi: 10.1023/a:1007772817842.
A clinically available mixture of hydroxyethylrutosides (HR) was examined as a protector against endothelial cell activation by hypoxia in perfused human umbilical vein. The results showed that 500 micrograms/mL HR totally inhibited the adherence of human unstimulated neutrophils to the endothelium of umbilical vein incubated in hypoxic conditions. This inhibition was confirmed by a morphological study performed by scanning electron microscopy. In addition, neutrophils adherent to the hypoxic umbilical vein endothelium became activated, as evidence by the increased release of superoxide anions and synthesis of leukotriene B4. These processes could also be inhibited by HR. In conclusion, the results of this study suggest that the improvement in venous insufficiency observed clinically with HR could, in part, be the result of their ability to inhibit the recruitment and activation of neutrophils by endothelium activated during blood stasis.
对一种临床上可用的羟乙基芦丁混合物（HR）进行了检测，以观察其作为灌注人脐静脉中缺氧诱导的内皮细胞活化的保护剂的效果。结果显示，500微克/毫升的HR完全抑制了在缺氧条件下孵育的人脐静脉内皮上未受刺激的人中性粒细胞的黏附。通过扫描电子显微镜进行的形态学研究证实了这种抑制作用。此外，黏附于缺氧脐静脉内皮的中性粒细胞被激活，这可通过超氧阴离子释放增加和白三烯B4合成增加得到证明。这些过程也能被HR抑制。总之，本研究结果表明，临床上观察到的HR对静脉功能不全的改善作用，部分可能是由于其能够抑制在血液淤滞期间被激活的内皮细胞对中性粒细胞的募集和激活。
