巴氏灭菌后白蛋白完整性的检测：一种使用蒽氧基脂肪酸类似物定量单体白蛋白的荧光测定法。
Checking the integrity of albumin after pasteurisation: a fluorescent assay for quantitating monomeric albumin using anthroyloxy-fatty acids analogs.
作者信息
Gallez B, Lambert D M
机构信息
Laboratory of Medicinal Chemistry and Radiopharmacy, Catholic University of Louvain, Brussels, Belgium.
出版信息
J Biochem Biophys Methods. 1998 Sep 24;37(1-2):5-9. doi: 10.1016/s0165-022x(98)00009-8.
PMID:9825296
Abstract
This study presents a new assay based on the binding of anthroyloxy-stearates to albumin and the change in the fluorescence intensity when these fluorescent probes are bound to the protein. This assay permits the quantification of the albumin monomers present in a mixture of monomers and polymers, and allows to check the integrity of albumin after pasteurisation.
摘要
本研究提出了一种基于蒽氧基硬脂酸盐与白蛋白结合以及这些荧光探针与蛋白质结合时荧光强度变化的新检测方法。该检测方法能够对单体和聚合物混合物中存在的白蛋白单体进行定量，并可检查巴氏杀菌后白蛋白的完整性。
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