Balabanov Iu V, Danilovskiĭ M A
Tsitologiia. 1976 Aug;18(8):985-9.
A technique of microinjection of exogenic commercial lactate dehydrogenase into the zygotes of rats has been elaborated. A selective inhibition of exogenous LDH-3, LDH-4 and LDH-5 was registered in the ova-recipients. Attempts to reproduce the selective inhibition of LDH-3, LDH-4 and LDH-5 by a microhomogenate made of zygotes were not successful. The identification of LDH from ovocytes and exogenic LDH was made according to their electrophoretic mobility relative to Bromphenol blue and LDH-1.
已研发出一种将外源性商业乳酸脱氢酶显微注射到大鼠受精卵中的技术。在卵受体中发现了对外源性乳酸脱氢酶-3、乳酸脱氢酶-4和乳酸脱氢酶-5的选择性抑制。试图通过由受精卵制成的微匀浆来重现对乳酸脱氢酶-3、乳酸脱氢酶-4和乳酸脱氢酶-5的选择性抑制未获成功。根据卵母细胞中的乳酸脱氢酶和外源性乳酸脱氢酶相对于溴酚蓝和乳酸脱氢酶-1的电泳迁移率对它们进行了鉴定。
