利用四重荧光STR分型系统（HUMVWA、HUMTH01、D21S11和HPRT）获得了两个中国汉族人群的遗传数据。

Genetic data obtained for two Chinese Han populations with a quadruplex fluorescent STR typing system (HUMVWA, HUMTH01, D21S11 and HPRT).

作者信息

Xiao F X, Gilissen A, Gu X X, Cassiman J J, Decorte R

机构信息

Center for Human Genetics, University of Leuven, Belgium.

出版信息

Int J Legal Med. 1998;111(6):343-5. doi: 10.1007/s004140050187.

DOI:10.1007/s004140050187

PMID:9826100

Abstract

Abstract DNA typing of four tetrameric repeat loci (HUMVWA, HUMTH0I, D21SII and HPRT) was carried out in a Chinese Han population from Shanghai (East China) and one from Guangzhou (South-East China) using a quadruplex PCR amplification and detection of the fluorescent-labeled alleles on the ALF DNA sequencer. All loci were in accordance with Hardy-Weinberg equilibrium except for D21S11 in the Guangzhou population. A test for population differentiation showed no statistical difference in the allele frequency distribution between the two populations. Comparison of the allele frequency data with other Chinese Han populations from North and South-West China for the STR loci HUMVWA and HUMTH01 revealed heterogeneity between Northern Chinese Han and Southern Chinese Han, which is in accordance with previous studies on the basis of protein markers.

摘要

摘要运用四重聚合酶链反应扩增，并在ALF DNA测序仪上对荧光标记的等位基因进行检测，对来自中国东部上海的一个汉族群体和来自中国东南部广州的一个汉族群体的四个四聚体重复基因座（HUMVWA、HUMTH0I、D21SII和HPRT）进行DNA分型。除广州群体中的D21S11外，所有基因座均符合哈迪-温伯格平衡。群体分化检验表明，两个群体之间的等位基因频率分布没有统计学差异。将STR基因座HUMVWA和HUMTH01的等位基因频率数据与来自中国北方和西南部的其他汉族群体进行比较，发现中国北方汉族和南方汉族之间存在异质性，这与之前基于蛋白质标记的研究结果一致。