Galich I P, Tsyperovich A S, Kolesnik L A, Tsesarskaia V D
Ukr Biokhim Zh. 1976 Jul-Aug;48(4):480-4.
Active immobilized alpha-amylase was obtained when applying AE-cellulose chromatography and glutaric dialdehyde. The time of the enzyme interaction with the carrier, amount of glutaric dialdehyde necessary for binding, optimal enzyme: carrier ratio as well as the methods for desiccation of the immobilized amylase preparations were specified. Conditions are selected for alpha-amylase stabilization with the presence of glutaric aldehyde. Immobilized amylase as compared to free enzyme is shown to be more pH-stable in the acid and alkaline zones of pH (2.0-3.5 and 10.5-12.0), thermostable (within a temperature range of 20-60 degrees C) and resistant to the effect of 5.5 M urea.
应用AE-纤维素色谱法和戊二醛可获得活性固定化α-淀粉酶。确定了酶与载体相互作用的时间、结合所需的戊二醛量、最佳酶与载体比例以及固定化淀粉酶制剂的干燥方法。选择了在戊二醛存在下α-淀粉酶稳定化的条件。与游离酶相比，固定化淀粉酶在pH值的酸性和碱性区域（2.0 - 3.5和10.5 - 12.0）表现出更高的pH稳定性、热稳定性（温度范围为20 - 60摄氏度）以及对5.5 M尿素作用的抗性。
