Kim J W, Kim S J, Han S M, Paik S Y, Hur S Y, Kim Y W, Lee J M, Namkoong S E
Department of Obstetrics and Gynecology, Research Institute of Molecular Genetics, Catholic University Medical College, 505 Banpo-dong, Seocho-ku, Seoul, 137-040, Korea.
Gynecol Oncol. 1998 Nov;71(2):266-9. doi: 10.1006/gyno.1998.5195.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a classical glycolytic protein. A higher level of GAPDH mRNA was found in lung, pancreas, and prostate cancers, but in cervical carcinoma there have not been any reports about the level of GAPDH gene expression. So, we tried to investigate the GAPDH gene expression patterns in cervical carcinomas compared to normal cervical tissues, and the relationships between the expression levels of this gene and conventional clinicopathological parameters were evaluated.
In this study, 25 normal exocervical tissues, 35 primary untreated cervical cancer tissues, 2 cervical cancer cell lines, and 2 post-nude-mouse-derived cervical cancer cell lines were subjected to Northern blot analyses for GAPDH gene expression.
Northern blot analyses revealed that the levels of GAPDH gene expression were elevated in 34 of 35 (97%) cervical carcinoma tissues and all of the 4 cervical cancer cell lines compared to normal cervical tissues. The levels of GAPDH gene expression were more prominent in rapidly proliferating cervical carcinoma cells. The levels of the GAPDH gene expressions in cervical cancer tissues were not associated with conventional clinicopathological parameters including clinical stage, histological type, and degree of differentiation.
These results suggest that increased GAPDH gene expression is characteristic of human cervical carcinomas and that rapidly proliferating carcinoma cells express more enhanced GAPDH gene. Future gene therapy using antisense oligodeoxynucleotide directed against GAPDH mRNA might be another therapeutic tool for human uterine cervical carcinoma.
甘油醛-3-磷酸脱氢酶（GAPDH）是一种经典的糖酵解蛋白。在肺癌、胰腺癌和前列腺癌中发现GAPDH mRNA水平较高，但在宫颈癌中尚未有关于GAPDH基因表达水平的报道。因此，我们试图研究与正常宫颈组织相比，宫颈癌中GAPDH基因的表达模式，并评估该基因表达水平与传统临床病理参数之间的关系。
在本研究中，对25份正常宫颈外组织、35份未经治疗的原发性宫颈癌组织、2种宫颈癌细胞系以及2种裸鼠传代后的宫颈癌细胞系进行GAPDH基因表达的Northern印迹分析。
Northern印迹分析显示，与正常宫颈组织相比，35份宫颈癌组织中的34份（97%）以及所有4种宫颈癌细胞系中GAPDH基因表达水平均升高。GAPDH基因表达水平在快速增殖的宫颈癌细胞中更为显著。宫颈癌组织中GAPDH基因的表达水平与包括临床分期、组织学类型和分化程度在内的传统临床病理参数无关。
这些结果表明，GAPDH基因表达增加是人类宫颈癌的特征，且快速增殖的癌细胞表达更强的GAPDH基因。未来使用针对GAPDH mRNA的反义寡脱氧核苷酸进行基因治疗可能是人类子宫颈癌的另一种治疗手段。
