Herrero M B, Cebral E, Franchi A, Motta A, Gimeno M F
Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Serrano 669, Buenos Aires, 1414, Argentina.
Biochem Biophys Res Commun. 1998 Nov 18;252(2):324-8. doi: 10.1006/bbrc.1998.9638.
In the present study we evidenced that progesterone could directly stimulate sperm nitric oxide synthase (NOS) in capacitated mouse spermatozoa. This stimulation led to an increase in sperm prostaglandin E2 (PGE2) production and subsequent acrosomal exocytosis. However, cGMP levels were not modified during the progesterone-induced acrosome reaction under our experimental conditions. These results suggest a functional role for nitric oxide as an intracellular messenger through, at least, stimulation of PGE2 production during the acrosome reaction triggered by progesterone.
在本研究中，我们证实孕酮可直接刺激获能小鼠精子中的一氧化氮合酶（NOS）。这种刺激导致精子前列腺素E2（PGE2）生成增加，并随后引发顶体胞吐作用。然而，在我们的实验条件下，孕酮诱导顶体反应期间cGMP水平未发生改变。这些结果表明，在孕酮引发的顶体反应过程中，一氧化氮至少通过刺激PGE2生成，作为一种细胞内信使发挥功能作用。
