Origuchi N, Ishidou Y, Nagamine T, Onishi T, Matsunaga S, Yoshida H, Sakou T
Department of Orthopaedic Surgery, Kagoshima University, Japan.
In Vivo. 1998 Sep-Oct;12(5):473-80.
In male rats and mice, the membranous bone is rapidly formed in the proximal segment (p-segment) of the os penis, and then endochondral ossification proceeds in the proximal half. The spatial and temporal patterns of expression of transforming growth factor-beta 1 (TGF-beta 1) and bone morphogentic protein-2/-4 (BMP-2/-4) were examined immunohistochemically until 14 days after birth and compared. BMP-2/-4 expression was weak in the mesenchymal condensation just at birth, and up-regulated in the membranous bone and cartilage 3 days after birth. BMP-2/-4 was strongly expressed in proliferating and mature chondrocytes 7 days after birth. At birth, TGF-beta 1 expression was diffuse and very strong in mesenchymal condensation. Thereafter, TGF-beta 1 expression was continuously strong in membranous bone and cartilage. Seven days after birth, TGF-beta 1 was actively expressed in proliferating chondrocytes. TGF-beta 1 and BMP-2/-4 seemed to act co-operatively. In particular, TGF-beta 1 appeared to strongly regulate immature stage and BMP-2/-4 may regulate the mature stage.
在雄性大鼠和小鼠中，阴茎骨近端段（p段）的膜内成骨迅速形成，然后近端一半进行软骨内成骨。通过免疫组织化学方法检测出生后14天内转化生长因子-β1（TGF-β1）和骨形态发生蛋白-2/-4（BMP-2/-4）的时空表达模式并进行比较。出生时，BMP-2/-4在间充质凝聚中的表达较弱，出生后3天在膜内骨和软骨中上调。出生后7天，BMP-2/-4在增殖和成熟软骨细胞中强烈表达。出生时，TGF-β1在间充质凝聚中表达弥漫且非常强烈。此后，TGF-β1在膜内骨和软骨中持续强烈表达。出生后7天，TGF-β1在增殖软骨细胞中活跃表达。TGF-β1和BMP-2/-4似乎协同发挥作用。特别是，TGF-β1似乎强烈调节未成熟阶段，而BMP-2/-4可能调节成熟阶段。
