Ferrer M, Sanz M L, Prieto I, Vila L, Oehling A
Department of Allergy and Clinical Immunology, University Clinic, Faculty of Medicine, University of Navarra, Spain.
J Investig Allergol Clin Immunol. 1998 Sep-Oct;8(5):277-80.
Numerous controlled trials have demonstrated the efficacy of specific immunotherapy, although its mechanism is not completely understood. Few studies have addressed the effects of immunotherapy on the release of mediators. We measured in vitro sulphidoleukotriene (sLT) and histamine release after specific stimulus (Dermatophagoides pteronyssinus or Lollium perenne) in a group of patients under immunotherapy (n = 35) and compared the results with those obtained in a group of allergic patients without immunotherapy (n = 57). SLT quantification was carried out by cellular stimulation allergen test (CAST)-ELISA and we measured the amount of histamine release using a fluorometric method. We found a significant (p < 0.05) reduction of allergen-specific mediator release on the group of patients under immunotherapy treatment. When we studied the group of patients sensitive to D. pteronyssinus we also observed a significant reduction in sLT release after the in vitro stimulus with anti-IgE. In vitro sLT production could be a good marker for follow-up immunotherapy. This study provides more evidence to support the immunological and cellular changes induced by immunotherapy.
大量对照试验已证明特异性免疫疗法的疗效，尽管其机制尚未完全明确。很少有研究探讨免疫疗法对介质释放的影响。我们对一组接受免疫疗法的患者（n = 35）在特定刺激（粉尘螨或黑麦草）后体外测量了硫代白三烯（sLT）和组胺的释放，并将结果与一组未接受免疫疗法的过敏患者（n = 57）的结果进行比较。通过细胞刺激过敏原试验（CAST）-ELISA进行sLT定量，我们使用荧光法测量组胺释放量。我们发现接受免疫疗法治疗的患者组中过敏原特异性介质释放显著减少（p < 0.05）。当我们研究对粉尘螨敏感的患者组时，在抗IgE体外刺激后也观察到sLT释放显著减少。体外sLT产生可能是免疫疗法随访的良好标志物。本研究提供了更多证据来支持免疫疗法诱导的免疫和细胞变化。
